抗Dectin-1/CLECSF12抗体(Anti-Dectin-1/CLECSF12, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Dectin-1/CLEC7A (R&D Systems, Catalog # 1859-DC)Thr66-Met201Accession # NP_072092
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Dectin-1/CLEC7A in direct ELISAs and Western blots. In this format, less than 2% cross‑reactivity with recombinant mouse Dectin-1 and recombinant human Dectin-2 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human Dectin‑1/CLEC7A (Catalog # 1859-DC)
Blockade of Receptor-ligand Interaction	In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Human Dectin-1/CLEC7A (Catalog # 1859-DC) coated at 250 ng/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

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Related Product & Information

Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Background	Dectin-1/CLEC7A
background_content	Background: Dectin-1/CLEC7A Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3-5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15-16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29-39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

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Citations

1. Osteopontin contributes to effective neutrophils recruitment, IL-1? production, and apoptosis in Aspergillus fumigatus keratitis
   Authors: G Zhao, M Hu, C Li, J Lee, K Yuan, G Zhu, C Che
   Immunol. Cell Biol., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan.
   Authors: Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N
   J Immunol, 2013;190(8):4116-28.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. The modular nature of dendritic cell responses to commensal and pathogenic fungi.
   Authors: Rizzetto, Lisa, Buschow, Sonja I, Beltrame, Luca, Figdor, Carl G, Schierer, Stephan, Schuler, Gerold, Cavalieri, Duccio
   PLoS ONE, 2012;7(8):e42430.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Protein kinase Cdelta is a critical component of Dectin-1 signaling in primary human monocytes.
   Authors: Elsori DH, Yakubenko VP, Roome T, Thiagarajan PS, Bhattacharjee A, Yadav SP, Cathcart MK
   J. Leukoc. Biol., 2011;90(3):599-611.
   Species: Human
   Sample Type: Whole Cells
   Application: WB


5. Costimulation of dectin-1 and DC-SIGN triggers the arachidonic acid cascade in human monocyte-derived dendritic cells.
   Authors: Valera I, Fernandez N, Trinidad AG, Alonso S, Brown GD, Alonso A, Crespo MS
   J. Immunol., 2008;180(8):5727-36.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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