抗Nucleostemin抗体(Anti-Nucleostemin, Human/Mouse/Rat, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant rat Nucleostemin Lys2-Ile538Accession # Q811S9
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, rat Nucleostemin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat Nucleostemin C-terminal peptide (aa 281-538) is observed. Rat Nucleostemin specific IgG was purified by first passing the sera over a rat Nucleostemin aa 2-538 column and then passing the bound fraction over a rat Nucleostemin aa 281-538 column to removed rat Nucleostemin C-terminal specific IgG.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Nucleostemin by Western Blot. Western blot shows lysates of PC‑3 human prostate cancer cell line and SW480 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nucleostemin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Nucleostemin in U2OS Human Cell Line. Nucleostemin was detected in immersion fixed U2OS human osteosarcoma cell line using 10 µg/mL Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Human Nucleostemin by Simple WesternTM. Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Nucleostemin at approximately 83 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Nucleostemin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1638) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background	Nucleostemin
background_content	Background: Nucleostemin Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells (1-3).

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Citations

1. Overexpression of nucleostemin contributes to an advanced malignant phenotype and a poor prognosis in oral squamous cell carcinoma.
   Authors: Yoshida, R, Nakayama, H, Nagata, M, Hirosue, A, Tanaka, T, Kawahara, K, Nakagawa, Y, Matsuoka, Y, Sakata, J, Arita, H, Hiraki, A, Shinohara, M, Ito, T
   Br J Cancer, 2014;111(12):2308-15.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Phenotypic plasticity in normal breast derived epithelial cells.
   Authors: Sauder C, Koziel J, Choi M, Fox M, Grimes B, Badve S, Blosser R, Radovich M, Lam C, Vaughan M, Herbert B, Clare S
   BMC Cell Biol, 2014;15(0):20.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Nucleostemin is indispensable for the maintenance and genetic stability of hematopoietic stem cells.
   Authors: Yamashita M, Nitta E, Nagamatsu G, Ikushima Y, Hosokawa K, Arai F, Suda T
   Biochem Biophys Res Commun, 2013;441(1):196-201.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Fresh


4. Reactive oxygen species regulate nucleostemin oligomerization and protein degradation.
   Authors: Huang M, Whang P, Chodaparambil JV
   J. Biol. Chem., 2011;286(13):11035-46.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Depletion of guanine nucleotides leads to the Mdm2-dependent proteasomal degradation of nucleostemin.
   Authors: Huang M, Itahana K, Zhang Y, Mitchell BS
   Cancer Res., 2009;69(7):3004-12.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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