抗LIF抗体(Anti-LIF, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse LIF
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than5% cross‑reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross‑reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse LIF
Neutralization	Measured by its ability to neutralize LIF-induced IL-6 secretion in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 10-60 ng/mL in the presence of 3 ng/mL Recombinant Mouse LIF.

Neutralization

IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND50 is typically 10-60 ng/mL.

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Related Product & Information

Long Name	Leukemia Inhibitory Factor
Entrez Gene IDs	3976 (Human); 16878 (Mouse); 403449 (Canine)
Background	LIF
background_content	Background: LIF Leukemia inhibitory factor (LIF) was initially identified as a factor that inhibited the proliferation and induced the differentiation to macrophages of the murine myeloid leukemic cell line M1. Subsequent to its purification and molecular cloning, LIF was recognized to be a pleiotropic factor with multiple effects on both hematopoietic and non-hematopoietic cells. LIF has overlapping biological functions with OSM, IL-6, IL-11 and CNTF. All these cytokines utilize gp130 as a component in their signal transducing receptor complexes. Mouse LIF cDNA encodes a 203 amino acid residue polypeptide with a 23 amino acid signal peptide that is cleaved to yield a 180 amino acid mature mouse LIF. Native human and mouse LIF are highly glycosylated monomeric proteins. Both human and murine LIF protein sequences have multiple potential N- and O-linked glycosylation sites and six conserved cysteine residues that are involved in three intramolecular disulfide bridges.

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Citations

1. TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs
   Authors: H Won, D Moreira, C Gao, P Duttagupta, X Zhao, E Manuel, D Diamond, YC Yuan, Z Liu, J Jones, M D'Apuzzo, S Pal, M Kortylewsk
   J. Leukoc. Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


2. Leukemia inhibitory factor signaling is required for lung protection during pneumonia.
   Authors: Quinton LJ, Mizgerd JP, Hilliard KL
   J. Immunol., 2012;188(12):6300-8.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity.
   Authors: Moidunny S, Vinet J
   J Neuroinflammation, 2012;9(1):198.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


4. Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease.
   Authors: Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, Zhang JZ
   Immunity, 2011;35(2):273-84.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. Astrocytes promote myelination in response to electrical impulses.
   Authors: Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD
   Neuron, 2006;49(6):823-32.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


6. The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
   Authors: Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
   Arch. Dermatol. Res., 2001;293(10):508-14.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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