抗DLL4抗体(Anti-DLL4, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse DLL4Ser28-Pro525Accession # BAB18580
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Mouse DLL4 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	DLL4 in bEnd.3 Mouse Cell Line. DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

DLL4

background_content

Background: DLL4 Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

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Citations

1. MPDZ promotes DLL4-induced Notch signaling during angiogenesis
   Authors: F Tetzlaff, MG Adam, A Feldner, I Moll, A Menuchin, J Rodriguez-, D Sprinzak, A Fischer
   Elife, 2018;7(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability
   Authors: AV Shah, GM Birdsey, C Peghaire, ME Pitulescu, NP Dufton, Y Yang, I Weinberg, L Osuna Alma, L Payne, JC Mason, H Gerhardt, RH Adams, AM Randi
   Nat Commun, 2017;8(0):16002.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


3. Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation
   Authors: RB Davis, DO Kechele, ES Blakeney, JB Pawlak, KM Caron
   JCI Insight, 2017;2(6):e92465.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Notch Signaling Regulates the Homeostasis of Tissue-Restricted Innate-like T Cells
   Authors: Vijaykumar Chennupati
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


5. Blood flow controls bone vascular function and osteogenesis
   Nat Commun, 2016;7(0):13601.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
   Authors: Zhou Y, Williams J, Smallwood P, Nathans J
   PLoS ONE, 2015;10(12):e0143650.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Frozen


7. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord.
   Authors: Zou M, Luo H, Xiang M
   Dev Dyn, 2015;244(1):86-97.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. Interleukin-6 Stimulates Defective Angiogenesis.
   Authors: Gopinathan G, Milagre C, Pearce O, Reynolds L, Hodivala-Dilke K, Leinster D, Zhong H, Hollingsworth R, Thompson R, Whiteford J, Balkwill F
   Cancer Res, 2015;75(15):3098-107.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
   Authors: Rama N, Dubrac A, Mathivet T, Ni Charthaigh R, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chedotal A
   Nat Med, 2015;21(5):483-91.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Not specified


10. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen

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