抗p53抗体(Anti-p53, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human p53Asp7-Asp393Accession # P04637
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat p53.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunoprecipitation	1-2 µg/500 µg cell lysate	CEM human T-lymphoblastoid cell line, see our available Western blot detection antibodies
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below
Knockout Validated	p53 is specifically detected in HeLa humancervical epithelial carcinoma parental cell line but is not detectable inp53 knockout HeLa cell line.

Western Blot
	Detection of Human p53 by Western Blot. Western blot shows lysates of CEM human T‑lymphoblastoid cell line and MCF‑7 human breast cancer cell line were mock-treated (-) or exposed (+) to 10 Gy ionizing radiation (IR) and harvested after 1 hour. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p53 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
	Detection of p53-regulated Genes by Chromatin Immunoprecipitation. MCF‑7 human breast cancer cell line treated with 300 nM camptothecin overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. p53/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Immunocytochemistry
	p53 in HeLa Human Cell Line. p53 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Human p53 by Simple WesternTM. Simple Western lane view shows lysates of CEM humanT-lymphoblastoid cell line and HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for p53 at approximately 59 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Knockout Validated
	Western Blot Shows Human p53 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat p53 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1355) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Entrez Gene IDs	7157 (Human); 22059 (Mouse); 24842 (Rat)
Background	p53
background_content	Background: p53 The p53 tumor suppressor protein is a multi-functional transcription factor that regulates cellular decisions regarding proliferation, cell cycle checkpoints, and apoptosis. The importance of p53 is underscored by its mutation in over 50% of human cancers. Mice that lack one or both copies of p53 also showed an increased incidence of tumors, which makes the p53 deficient mouse a model system for studying cancer generation and progression.

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Citations

1. B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
   Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
   Oncol Lett, 2018;15(6):9009-9016.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


2. Chromosomal instability induced by increased BIRC5/Survivin levels affects tumorigenicity of glioma cells
   Authors: M Conde, S Michen, R Wiedemuth, B Klink, E Schröck, G Schackert, A Temme
   BMC Cancer, 2017;17(1):889.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Dysfunction of the MDM2/p53 axis is linked to premature aging
   Authors: D Lessel, D Wu, C Trujillo, T Ramezani, I Lessel, MK Alwasiyah, B Saha, FM Hisama, K Rading, I Goebel, P Schütz, G Speit, J Högel, H Thiele, G Nürnberg, P Nürnberg, M Hammerschm, Y Zhu, DR Tong, C Katz, GM Martin, J Oshima, C Prives, C Kubisch
   J. Clin. Invest., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Growth hormone is permissive for neoplastic colon growth
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Combining anti-miR-155 with chemotherapy for the treatment of lung cancers
   Authors: Katrien Van Roosbr
   Clin. Cancer Res, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53.
   Authors: Wiedemuth R, Klink B, Topfer K, Schrock E, Schackert G, Tatsuka M, Temme A
   Mol Cancer, 2014;13(0):107.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha.
   Authors: Tsoporis JN, Izhar S, Parker TG
   J. Biol. Chem., 2008;283(44):30174-83.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


8. Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon.
   Authors: Ishida T, Sakaguchi I,
   Biol. Pharm. Bull., 2007;30(5):928-34.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. TIMP-1 gene deficiency increases tumour cell sensitivity to chemotherapy-induced apoptosis.
   Authors: Davidsen ML, Wurtz SØ, Romer MU, Sorensen NM, Johansen SK, Christensen IJ, Larsen JK, Offenberg H, Brunner N, Lademann U
   Br. J. Cancer, 2006;95(8):1114-20.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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