抗MIF抗体(Anti-MIF, Human, Goat-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：28845
MIFに対する抗体(Anti-MIF, Human, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
カートに商品を
追加しました。
追加しました。
価格
[在庫・価格 ：2026年04月23日 10時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
| 詳細 | 商品名 |
|
文献数 | ||
|---|---|---|---|---|---|
|
Anti-MIF, Human, Goat-Poly |
|
29 | |||
|
Anti-Human MIF Affinity Purified Polyclonal Ab |
|
13 | |||
[在庫・価格 ：2026年04月23日 10時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-MIF, Human, Goat-Poly
文献数： 29
- 商品コード：AF-289-PB
- メーカー：RSD
- 包装：100μg
- 価格：¥83,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
Anti-Human MIF Affinity Purified Polyclonal Ab
文献数： 13
- 商品コード：AF-289-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥27,000
- 在庫：1個
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
カートに商品を
追加しました。
追加しました。
Product Details
| Species Reactivity | Human, Mouse, Rat |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human MIFPro2-Ala115Accession # AAA36315 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human, mouse, and rat MIF in Western blots. |
カートに商品を
追加しました。
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.5 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Intracellular Staining by Flow Cytometry | 0.25 µg/106 cells | HL‑60 human acute promyelocytic leukemia cell line treated with Camphtothecin, fixed with paraformaldehyde, and permeabilized with saponin |
| Western Blot | |
|---|---|
 | Detection of Human MIF by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
| Western Blot | |
 | Detection of Mouse and Rat MIF by Western Blot. Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat MIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-289-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MIF at approximately 12-14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
カートに商品を
追加しました。
追加しました。
Related Product & Information
| Background | MIF |
|---|---|
| background_content | Background: MIF MIF (or macrophage migration inhibitory factor) was the first lymphokine/cytokine to be recognized in the pregenomics era (1, 2). Regardless, it is one of the least understood of all inflammatory mediators (1, 3). Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without a signal sequence (4-7). Secretion occurs non-classically via an ABCA1 transporter (8). The initiating Met is removed, leaving Pro as the first amino acid. The molecule consists of two alpha -helices and six beta -strands, four of which form a beta -sheet. The two remaining beta -strands interact with other MIF molecules, creating a trimer (2, 9, 10). Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position #1 (11). Amino acids 50-65 have also been suggested to contain thiol-protein oxidoreductase activity (12). MIF has proinflammatory cytokine activity centered around aa’s 49-65. On fibroblasts, MIF induces, IL-1, IL-8, and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release following IFN-gamma activation (13, 14). MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction (15, 16). Human MIF is active on mouse cells (14). Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine, and rat MIF, respectively. |
カートに商品を
追加しました。
追加しました。
Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
Application: Affinity Assay - MIF inhibits monocytic movement through a non-canonical receptor and disruption of temporal Rho GTPase activities in U-937 cells.
Authors: DiCosmo-Ponticello C, Hoover D, Coffman F, Cohen S, Cohen M
Cytokine, 2014;69(1):47-55.
Species: Human
Sample Type: Cell Lysates
Application: WB - Effect of macrophage migration inhibitory factor (MIF) in human placental explants infected with Toxoplasma gondii depends on gestational age.
Authors: de Oliveira Gomes A, de Oliveira Silva DA, Silva NM, de Freitas Barbosa B, Franco PS, Angeloni MB, Fermino ML, Roque-Barreira MC, Bechi N, Paulesu LR, Dos Santos MC, Mineo JR, Ferro EA
Am. J. Pathol., 2012;178(6):2792-801.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Human liver stem cell-derived microvesicles inhibit hepatoma growth in SCID mice by delivering antitumor microRNAs.
Authors: Fonsato V, Collino F, Herrera M, Cavallari C, Deregibus M, Cisterna B, Bruno S, Romagnoli R, Salizzoni M, Tetta C, Camussi G
Stem Cells, 2012;30(9):1985-98.
Species: Human
Sample Type: Whole Cells
Application: IHC Paraffin-embedded - Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis.
Authors: Herrero LJ, Nelson M, Srikiatkhachorn A, Gu R, Anantapreecha S, Fingerle-Rowson G, Bucala R, Morand E, Santos LL, Mahalingam S
Proc. Natl. Acad. Sci. U.S.A., 2011;108(29):12048-53.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
Authors: Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL
Cancer Lett., 2010;290(1):49-57.
Species: Human
Sample Type: Serum
Application: WB - Human plasma membrane-derived vesicles halt proliferation and induce differentiation of THP-1 acute monocytic leukemia cells.
Authors: Ansa-Addo EA, Lange S, Stratton D, Antwi-Baffour S, Cestari I, Ramirez MI, McCrossan MV, Inal JM
J. Immunol., 2010;185(9):5236-46.
Species: Human
Sample Type: Whole Cells
Application: Flow - Sex dimorphism in wound healing: the roles of sex steroids and macrophage migration inhibitory factor.
Authors: Gilliver SC, Ruckshanthi JP, Hardman MJ, Nakayama T, Ashcroft GS
Endocrinology, 2008;149(11):5747-57.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB - Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha.
Authors: Daryadel A, Grifone RF, Simon HU, Yousefi S
J. Biol. Chem., 2006;281(37):27653-61.
Species: Human
Sample Type: Cell Lysates
Application: WB - Variation in macrophage-migration-inhibitory-factor immunoreactivity during porcine gestation.
Authors: Paulesu L, Cateni C, Romagnoli R, Ietta F, Dantzer V
Biol. Reprod., 2005;72(4):949-53.
Species: Human
Sample Type: Tissue Homogenates
Application: WB
カートに商品を
追加しました。
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。