抗Sca-1抗体(Anti-Sca-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E alleleLeu27-Gly119Accession # CAA28351
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Sca-1/Ly6 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Sca‑1/Ly6
Flow Cytometry	2.5 µg/106 cells	Mouse splenocytes
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry

Sca‑1/Ly6 in Mouse Splenocytes. Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Goat Anti-Mouse Sca‑1/Ly6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Stem Cell Antigen-1/Lymphocyte Antigen 6
Entrez Gene IDs	110454 (Mouse)
Background	Sca-1/Ly6
background_content	Background: Sca-1/Ly6 This antibody reacts with Sca-1, an 18 kDa phosphatidylinositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly‑6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non‑hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

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Citations

1. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress
   Authors: E Zhu, L Hu, H Wu, L Piao, G Zhao, A Inoue, W Kim, C Yu, W Xu, YK Bando, X Li, Y Lei, CN Hao, K Takeshita, WS Kim, K Okumura, T Murohara, M Kuzuya, XW Cheng
   J Am Heart Assoc, 2017;6(7):.
   


3. Bone marrow-derived cells and their conditioned medium induce microvascular repair in uremic rats by stimulation of endogenous repair mechanisms
   Authors: L Golle, HU Gerth, K Beul, B Heitplatz, P Barth, M Fobker, H Pavenstädt, GS Di Marco, M Brand
   Sci Rep, 2017;7(1):9444.
   Species: Rat
   Sample Type: Whole Cells
   Application: Flow


4. Systemic application of sirolimus prevents neointima formation not via a direct anti-proliferative effect but via its anti-inflammatory properties
   Authors: JM Daniel, J Dutzmann, H Brunsch, J Bauersachs, R Braun-Dull, DG Sedding
   Int. J. Cardiol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Pluripotent stem cells derived from mouse and human white mature adipocytes.
   Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
   Stem Cells Transl Med, 2014;3(2):161-71.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Age dependence of lung mesenchymal stromal cell dynamics following pneumonectomy.
   Authors: Paxson J, Gruntman A, Davis A, Parkin C, Ingenito E, Hoffman A
   Stem Cells Dev, 2013;22(24):3214-25.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
   Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
   Nature, 2013;504(7479):277-81.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Three-dimensional culture of mouse renal carcinoma cells in agarose macrobeads selects for a subpopulation of cells with cancer stem cell or cancer progenitor properties.
   Authors: Smith BH, Gazda LS, Conn BL
   Cancer Res., 2011;71(3):716-24.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Structural basis of digoxin that antagonizes RORgamma t receptor activity and suppresses Th17 cell differentiation and interleukin (IL)-17 production.
   Authors: Fujita-Sato S, Ito S, Isobe T, Ohyama T, Wakabayashi K, Morishita K, Ando O, Isono F
   J. Biol. Chem., 2011;286(36):31409-17.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. Stromal cell-derived factor-1 enhances distraction osteogenesis-mediated skeletal tissue regeneration through the recruitment of endothelial precursors.
    Authors: Fujio M, Yamamoto A, Ando Y, Shohara R, Kinoshita K, Kaneko T, Hibi H, Ueda M
    Bone, 2011;49(4):693-700.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen

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