抗TNF Receptor Ⅰ抗体(Anti-TNF Receptor Ⅰ, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human sTNF RI/TNFRSF1AAccession # NP_001056
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 10% cross-reactivity with recombinant mouse TNF sRI is observed. Additionally, in direct ELISAs, this antibody does not cross-react with recombinant human (rh) sTNF RII, recombinant mouse (rm) sTNF RII, rhTNF‑ alpha, rmTNF‑ alpha, rhTNF‑ beta. This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L‑929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human TNF RI/TNFRSF1A (Catalog # 636-R1)
Flow Cytometry	2.5 µg/106 cells	Human blood-derived monocytes
Agonist Activity	Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-6 μg/mL.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes. TNF RI/TNFRSF1A was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Human TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF225) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

目次に戻る

Related Product & Information

Long Name	Tumor Necrosis Factor Receptor I
Entrez Gene IDs	7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Background	TNF RI/TNFRSF1A
background_content	Background: TNF RI/TNFRSF1A TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

目次に戻る

Citations

1. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
   Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F
   PLoS ONE, 2015;10(12):e0143224.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC-Frozen


2. Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor kappaB (NF-kappaB) signaling.
   Authors: Wang Y, Tan B, Mu R, Chang Y, Wu M, Tu H, Zhang Y, Guo S, Qin X, Li T, Li W, Li A, Zhang X, Li H
   J Biol Chem, 2015;290(16):10395-405.
   Species: Human
   Sample Type: Whole Cells
   Application: IP


3. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
   Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
   J. Immunol. Methods, 2012;378(1):72-80.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


4. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
   Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
   Dig. Dis. Sci., 2007;52(10):2775-83.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
   Authors: Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF
   Atherosclerosis, 2007;192(1):25-32.
   Species: Porcine
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

目次に戻る