抗Adiponectin抗体(Anti-Adiponectin, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Adiponectin/Acrp30 (R&D Systems, Catalog # 5095-AC)Glu18-Asn247Accession # Q60994
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, greater than 50% cross-reactivity with recombinant rat Adiponectin is observed and less than 30% cross-reactivity with recombinant human Adiponectin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Adiponectin/Acrp30 (Catalog # 5095-AC)
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	Adiponectin/Acrp30 in ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed ST-2 mouse bone marrow-derived stromal cell line differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Adiponectin/Acrp30 in human mesenchymal stem cells differentiated to adipocytes. Adiponectin/Acrp30 was detected in immersion fixed human mesenchymal stem cells differentiated to adipocytes using Goat Anti-Mouse Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Adiponectin/Acrp30

background_content

Background: Adiponectin/Acrp30 Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature mouse adiponectin consists of a 66 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-10). Mature mouse adiponectin shares 83% and 91% amino acid (aa) sequence identity with human and rat adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into >300 kDa high molecular weight (HMW) oligomers (11-13). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (14). The various multimeric forms of adiponectin exhibit distinct tissue specific and gender specific profiles and activities (13, 15).

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Citations

1. Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release
   Authors: Y Obata, S Kita, Y Koyama, S Fukuda, H Takeda, M Takahashi, Y Fujishima, H Nagao, S Masuda, Y Tanaka, Y Nakamura, H Nishizawa, T Funahashi, B Ranscht, Y Izumi, T Bamba, E Fukusaki, R Hanayama, S Shimada, N Maeda, I Shimomura
   JCI Insight, 2018;3(8):.
   Species: Mouse
   Sample Type:
   


2. The unique prodomain of T-cadherin plays a key role in adiponectin binding with the essential extracellular cadherin repeats 1 and 2
   Authors: S Fukuda, S Kita, Y Obata, Y Fujishima, H Nagao, S Masuda, Y Tanaka, H Nishizawa, T Funahashi, J Takagi, N Maeda, I Shimomura
   J. Biol. Chem, 2017;0(0):.
   Species: Mouse
   Sample Type: Serum
   Application: WB


3. Retinol-binding protein 7 is an endothelium-specific PPAR? cofactor mediating an antioxidant response through adiponectin
   Authors: C Hu, HL Keen, KT Lu, X Liu, J Wu, DR Davis, SC Ibeawuchi, S Vogel, FW Quelle, CD Sigmund
   JCI Insight, 2017;2(6):e91738.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Role of the adipose PPAR?-adiponectin axis in susceptibility to stress and depression/anxiety-related behaviors
   Mol. Psychiatry, 2016;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice
   Authors: M C Muñoz
   Peptides, 2016;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


6. Positive feedback regulation between adiponectin and T-cadherin impacts adiponectin levels in tissue and plasma of male mice.
   Authors: Matsuda, Keisuke, Fujishima, Yuya, Maeda, Norikazu, Mori, Takuya, Hirata, Ayumu, Sekimoto, Ryohei, Tsushima, Yu, Masuda, Shigeki, Yamaoka, Masaya, Inoue, Kana, Nishizawa, Hitoshi, Kita, Shunbun, Ranscht, Barbara, Funahashi, Tohru, Shimomura, Iichiro
   Endocrinology, 2015;156(3):934-46.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Ultrastructural localization of adiponectin protein in vasculature of normal and atherosclerotic mice.
   Authors: Mori T, Koyama Y, Maeda N, Nakamura Y, Fujishima Y, Matsuda K, Funahashi T, Shimada S, Shimomura I
   Sci Rep, 2014;4(0):4895.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


8. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
   Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
   Nature, 2013;504(7479):277-81.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. T-cadherin is essential for adiponectin-mediated revascularization.
   Authors: Parker-Duffen, Jennifer, Nakamura, Kazuto, Silver, Marcy, Kikuchi, Ryosuke, Tigges, Ulrich, Yoshida, Sumiko, Denzel, Martin S, Ranscht, Barbara, Walsh, Kenneth
   J Biol Chem, 2013;288(34):24886-97.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: WB


10. Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
    Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
    Cytokine, 2013;62(2):206-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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