抗SOX2抗体（Anti-SOX2, Goat-Poly antibody）

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	E. coli-derived recombinant human SOX2Gly135-Met317Accession # P48431
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human SOX2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX1 and rhSOX17 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	5 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
	Detection of SOX2-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Immunocytochemistry
	SOX2 in Mouse Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected in stem cells using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and co-stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunocytochemistry
	SOX2 in Rat Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Simple Western
	Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 53 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunocytochemistry
	SOX2 in ADLF1 and FAB2 Stem Cell Lines. SOX2 was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

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Related Product & Information

Background	SOX2
background_content	Background: SOX2 SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).

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Citations

1. SNAI2 upregulation is associated with an aggressive phenotype in fulvestrant-resistant breast cancer cells and is an indicator of poor response to endocrine therapy in estrogen receptor-positive metastatic breast cancer
   Authors: CL Alves, D Elias, MB Lyng, M Bak, HJ Ditzel
   Breast Cancer Res., 2018;20(1):60.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. NMDA receptor signaling is important for neural tube formation and for preventing antiepileptic drug-induced neural tube defects
   Authors: EB Sequerra, R Goyal, PA Castro, JB Levin, LN Borodinsky
   J. Neurosci., 2018;0(0):.
   Species: Xenopus
   Sample Type: Whole Tissue
   Application: IHC


3. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium
   Authors: TJ Lynch, PJ Anderson, PG Rotti, SR Tyler, AK Crooke, SH Choi, DT Montoro, CL Silverman, W Shahin, R Zhao, CW Jensen-Cod, A Adamcakova, TIA Evans, W Xie, Y Zhang, H Mou, BP Herring, PS Thorne, J Rajagopal, C Yeaman, KR Parekh, JF Engelhardt
   Cell Stem Cell, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. NADsupplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency
   Authors: Y Hou, S Lautrup, S Cordonnier, Y Wang, DL Croteau, E Zavala, Y Zhang, K Moritoh, JF O'Connell, BA Baptiste, TV Stevnsner, MP Mattson, VA Bohr
   Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
   Authors: J Belkind-Ge, HK Graham, J Reynolds, R Hotta, N Nagy, L Cheng, M Kamionek, HN Shi, CM Aherne, AM Goldstein
   Sci Rep, 2017;7(1):2525.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Evidence of functional duplicity of Nestin expression in the adult mouse midbrain
   Authors: P Farzanehfa, SS Lu, A Dey, D Musiienko, H Baagil, MK Horne, TD Aumann
   Stem Cell Res, 2017;19(0):82-93.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Human neural progenitors derived from integration-free iPSCs for SCI therapy
   Authors: Y Liu, Y Zheng, S Li, H Xue, K Schmitt, GW Hergenroed, J Wu, Y Zhang, DH Kim, Q Cao
   Stem Cell Res, 2017;19(0):55-64.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Reduced expression of the murine HLA-G homolog Qa-2 is associated with malignancy, epithelial-mesenchymal transition and stemness in breast cancer cells
   Authors: IL da Silva, L Montero-Mo, E Martín-Vil, J Martin-Pér, B Sainz, J Renart, R Toscano Si, É Soares Vel, C Salviano T, MC de Oliveir, E Ferreira, M Quintanill
   Sci Rep, 2017;7(1):6276.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Peptide-Based Scaffolds Support Human Cortical Progenitor Graft Integration to Reduce Atrophy and Promote Functional Repair in a Model of Stroke
   Authors: FA Somaa, TY Wang, JC Niclis, KF Bruggeman, JA Kauhausen, H Guo, S McDougall, RJ Williams, DR Nisbet, LH Thompson, CL Parish
   Cell Rep, 2017;20(8):1964-1977.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts
    Authors: CR Ball, F Oppel, KR Ehrenberg, TD Dubash, SM Dieter, CM Hoffmann, U Abel, F Herbst, M Koch, J Werner, F Bergmann, N Ishaque, M Schmidt, C von Kalle, C Scholl, S Fröhling, B Brors, W Weichert, J Weitz, H Glimm
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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