抗B220/CD45R抗体(Anti-B220, Mouse, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information

価格

目次に戻る

Product Details

Species Reactivity	Mouse
Label	Unconjugated
Source	Monoclonal Rat IgG2a Clone # RA3-6B2
Specificity	This antibody has been shown to react with the extracellular domains of a subset of mouse CD45 isoforms predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells.1 - 3 The level of B220 antigen expression on the B cell lineage is developmentally regulated 2 - 4 and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses.5, 6

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Cell depletion	This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete B220+ cells from 1 x 109 bone marrow cells.

Cell Depletion
	Cell Depletion Figure 1: Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.
Cell Depletion
	Cell Depletion
Cell Depletion
	Cell Depletion

目次に戻る

Related Product & Information

Background	B220/CD45R
background_content	Background: B220/CD45R B220 antigens represent a subset of mouse CD45 isoforms predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on the B cell lineage is developmentally regulated and this antibody is commonly used as a B cell marker.

目次に戻る