抗MIP-1β抗体(Anti-MIP-1β, Human, Mouse-Mono, FITC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta Ala24-Asn92Accession # P13236
Source	Monoclonal Mouse IgG2B Clone # 24006
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP‑1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP‑1 alpha, rmCCL4/MIP‑1 beta, rhCXCL8/IL‑8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of CCL4/MIP‑1 beta in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human CCL4/MIP‑1 beta Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC271F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Entrez Gene IDs	6351 (Human); 20303 (Mouse); 116637 (Rat); 448786 (Canine)
Background	CCL4/MIP-1 beta
background_content	Background: CCL4/MIP-1 beta CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ) is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

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Citations

1. Preservation of Lymphopoietic Potential and Virus Suppressive Capacity by CD8+ T Cells in HIV-2-Infected Controllers
   J Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Human CD8+ T-cells recognizing peptides from Mycobacterium tuberculosis (Mtb) presented by HLA-E have an unorthodox Th2-like, multifunctional, Mtb inhibitory phenotype and represent a novel human T-cell subset.
   Authors: van Meijgaarden K, Haks M, Caccamo N, Dieli F, Ottenhoff T, Joosten S
   PLoS Pathog, 2015;11(3):e1004671.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. CD8+ regulatory T cells, and not CD4+ T cells, dominate suppressive phenotype and function after in vitro live Mycobacterium bovis-BCG activation of human cells.
   Authors: Boer M, van Meijgaarden K, Joosten S, Ottenhoff T
   PLoS ONE, 2014;9(4):e94192.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. CD39 is involved in mediating suppression by Mycobacterium bovis BCG-activated human CD8(+) CD39(+) regulatory T cells.
   Authors: Boer M, van Meijgaarden K, Bastid J, Ottenhoff T, Joosten S
   Eur J Immunol, 2013;43(7):1925-32.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. A molecular basis for the control of preimmune escape variants by HIV-specific CD8+ T cells.
   Authors: Ladell K, Hashimoto M, Iglesias M, Wilmann P, McLaren J, Gras S, Chikata T, Kuse N, Fastenackels S, Gostick E, Bridgeman J, Venturi V, Arkoub Z, Agut H, van Bockel D, Almeida J, Douek D, Meyer L, Venet A, Takiguchi M, Rossjohn J, Price D, Appay V
   Immunity, 2013;38(3):425-36.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope.
   Authors: Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R
   PLoS Pathog, 2012;8(11):e1003042.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Early Induction of Polyfunctional Simian Immunodeficiency Virus (SIV)-Specific T Lymphocytes and Rapid Disappearance of SIV from Lymph Nodes of Sooty Mangabeys during Primary Infection.
   Authors: Meythaler M, Wang Z, Martinot A, Pryputniewicz S, Kasheta M, McClure HM, O'Neil SP, Kaur A
   J. Immunol., 2011;186(9):5151-61.
   Species: Primate - Cercocebus torquatus (Sooty Mangabey)
   Sample Type: Whole Cells
   Application: Flow


8. CD16- natural killer cells: enrichment in mucosal and secondary lymphoid tissues and altered function during chronic SIV infection.
   Authors: Reeves RK, Gillis J, Wong FE
   Blood, 2010;115(22):4439-46.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Cells
   Application: Flow


9. Interleukin-27 induces a STAT1/3- and NF-kappaB-dependent proinflammatory cytokine profile in human monocytes.
   Authors: Guzzo C, Che Mat NF, Gee K
   J. Biol. Chem., 2010;285(32):24404-11.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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