抗Angiotensin Converting Enzyme 2抗体(Anti-Angiotensin Converting Enzyme 2, Ectodomain, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human ACE-2Gln18-Ser740 (predicted)Accession # Q9BYF1
Source	Monoclonal Mouse IgG2A Clone # 171606
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human ACE-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ACE-1 or rhNeprilysin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of human kidney

Western Blot

Detection of Human ACE‑2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB933) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ACE‑2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Background

ACE-2

background_content

Background: ACE-2 Angiogensin I Converting Enzyme-2 (ACE-2), also called ACEH (ACE homolog), is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function (1). ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). ACE-2 isoforms of 75 kDa and 120 kDa are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage.

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Citations

1. Angiotensin-converting enzyme 2 is a potential therapeutic target for EGFR-mutant lung adenocarcinoma
   Authors: M Yamaguchi, S Hirai, T Sumi, Y Tanaka, M Tada, Y Nishii, T Hasegawa, H Uchida, G Yamada, A Watanabe, H Takahashi, Y Sakuma
   Biochem. Biophys. Res. Commun., 2017;0(0):.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


2. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein.
   Authors: Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pohlmann S
   J Virol, 2014;88(2):1293-307.
   Species: Human
   Sample Type: Cell Lysates
   Application: Functional Assay


3. Selective and specific regulation of ectodomain shedding of angiotensin-converting enzyme 2 by tumor necrosis factor alpha-converting enzyme.
   Authors: Iwata M, Silva Enciso JE, Greenberg BH
   Am. J. Physiol., Cell Physiol., 2009;297(5):C1318-29.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis.
   Authors: Lovren F, Pan Y, Quan A, Teoh H, Wang G, Shukla PC, Levitt KS, Oudit GY, Al-Omran M, Stewart DJ, Slutsky AS, Peterson MD, Backx PH, Penninger JM, Verma S
   Am. J. Physiol. Heart Circ. Physiol., 2008;295(4):H1377-84.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. JNK and PI3k/Akt signaling pathways are required for establishing persistent SARS-CoV infection in Vero E6 cells.
   Authors: Mizutani T, Fukushi S, Saijo M, Kurane I, Morikawa S
   Biochim. Biophys. Acta, 2005;1741(1):4-10.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor.
   Authors: Hofmann H, Geier M, Marzi A, Krumbiegel M, Peipp M, Fey GH, Gramberg T, Pohlmann S
   Biochem. Biophys. Res. Commun., 2004;319(4):1216-21.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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