抗Human BMP-4抗体(Anti-Human BMP-4 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human BMP‑4Ser293-Arg408Accession # P12644
Source	Monoclonal Mouse IgG2B Clone # 66119
Purification	Protein A or G purified from ascites
Specificity	Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP‑2.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human BMP‑4 (Catalog # 314-BP)
Neutralization	Measured by its ability to neutralize BMP‑4-induced alkaline phosphatase production in the C2C12 mouse myoblast cell line. Katagiri, T. et al. (1994) J. Cell Biol. 127:1755. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 500 ng/mL Recombinant Human BMP‑4 and 2 µg/mL heparin.

Neutralization

Alkaline Phosphatase Production Induced by BMP‑4 and Neutralization by Human BMP‑4 Antibody. Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).

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Related Product & Information

Long Name	Bone Morphogenetic Protein 4
Entrez Gene IDs	652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Background	BMP-4
background_content	Background: BMP-4 BMP4 is a TGF beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 5). The human BMP4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfidelinked homodimeric BMP4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfidelinked heterodimer (7 9). Mature human and mouse BMP4 share 98% aa sequence identity. Human BMP4 shares 85% aa sequence identity with human BMP2 and less than 50% with other human BMPs. Compared to BMP4 homodimers, BMP4/ BMP7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPRIA) and type II (primarily Activin RIIA or BMPRII) receptors (11, 12). The bioavailability of BMP4 is regulated by its interaction with multiple proteins and glycosaminoglycan (13 - 15).

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Citations

1. Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells
   Authors: C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat
   J. Biol. Chem., 2017;0(0):.
   Species: Human
   Sample Type: Recombinant Proein
   Application: IP


2. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
   Authors: Herrera B, Inman GJ,
   BMC Cell Biol., 2009;10(0):20.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Neut


3. Chordin-like 1, a bone morphogenetic protein-4 antagonist, is upregulated by hypoxia in human retinal pericytes and plays a role in regulating angiogenesis.
   Authors: Kane R, Godson C, O'Brien C
   Mol. Vis., 2008;14(0):1138-48.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
   Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
   Blood, 2008;112(8):3154-63.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells.
   Authors: Zode GS, Clark AF, Wordinger RJ
   Invest. Ophthalmol. Vis. Sci., 2007;48(11):5058-67.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


6. Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions.
   Authors: Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH
   Ann. Neurol., 2007;62(3):288-300.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
   Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR
   J. Pathol., 2006;209(2):190-7.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation.
   Authors: Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C
   Mol. Cell. Biol., 2003;23(16):5664-79.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Neut


9. Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
   Authors: van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
   Gastroenterology, 2001;121(2):317-28.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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