抗Human TIM-1/KIM-1/HAVCR抗体(Anti-Human TIM-1/KIM-1/HAVCR antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCRSer21-Thr288Accession # AAC39862
Source	Monoclonal Mouse IgG2B Clone # 219211
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human TIM‑1/KIM-1/HAVCR in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) TIM-1, rmTIM-2, or rhTIM-3.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	8-25 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Western Blot
	Detection of Human TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of human CD4+ cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (Catalog # MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TIM‑1/KIM‑1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Flow Cytometry
	Detection of TIM‑1/KIM‑1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. (A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Immunohistochemistry
	TIM‑1/KIM‑1/HAVCR in Human Kidney. TIM‑1/KIM‑1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Related Product & Information

Background

TIM-1/KIM-1/HAVCR

background_content

Background: TIM-1/KIM-1/HAVCR TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms ofTIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85 - 90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).

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Citations

1. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
   Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
   Physiol Rep, 2018;6(6):e13654.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


2. TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions
   Authors: A Das, A Hirai-Yuki, O González-L, B Rhein, S Moller-Tan, R Brouillett, L Hensley, I Misumi, W Lovell, JM Cullen, JK Whitmire, W Maury, SM Lemon
   MBio, 2017;8(5):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
   Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
   Sci Rep, 2017;7(0):41389.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. Attachment and Post-Attachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission
   Authors: H Fan, L Qiao, KD Kang, J Fan, W Wei, G Luo
   J. Virol., 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Expression of Tim-1 in primary CNS lymphoma
   Cancer Med, 2016;5(11):3235-3245.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


6. TIM-1 promotes hepatitis C virus cell attachment and infection
   Authors: Guangxiang Luo
   J. Virol., 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection.
   Authors: Kuroda M, Fujikura D, Noyori O, Kajihara M, Maruyama J, Miyamoto H, Yoshida R, Takada A
   Biochem Biophys Res Commun, 2014;455(3):223-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Acute pretreatment with chloroquine attenuates renal I/R injury in rats.
   Authors: Todorovic, Zoran, Medic, Branisla, Basta-Jovanovic, Gordana, Radojevic Skodric, Sanja, Stojanovic, Radan, Rovcanin, Branisla, Prostran, Milica
   PLoS ONE, 2014;9(3):e92673.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease.
   Authors: Wilkinson R, Wang X, Kassianos A, Zuryn S, Roper K, Osborne A, Sampangi S, Francis L, Raghunath V, Healy H
   PLoS ONE, 2014;9(1):e87345.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


10. TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.
    Authors: Jemielity, Stephani, Wang, Jinyize, Chan, Ying Kai, Ahmed, Asim A, Li, Wenhui, Monahan, Sheena, Bu, Xia, Farzan, Michael, Freeman, Gordon J, Umetsu, Dale T, Dekruyff, Rosemari, Choe, Hyeryun
    PLoS Pathog, 2013;9(3):e1003232.
    

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