抗iNOS抗体(Anti-iNOS, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Recombinant human iNOSPro781-His798Accession # P35228
Source	Monoclonal Mouse IgG1 Clone # 2D2-B2
Purification	Protein A or G purified from ascites
Specificity	Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	See below

Western Blot
	Detection of Human iNOS by Western Blot. Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog #201‑LB, 210‑TA, 285‑IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Western Blot
	Detection of Mouse and Rat iNOS by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iNOS at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	iNOS in Human Brain. iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Mouse iNOS by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with LPS, loaded at 0.2 mg/mL. A specific band was detected for iNOS at approximately 136 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Inducible Nitic Oxide Synthase
Background	iNOS
background_content	Background: iNOS Inducible nitric oxide synthase (iNOS) is a widely expressed, approximately 130 kDa enzyme that catalyzes the conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a bioactive mediator that plays an important role in hemodynamics by inducing vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney. In other tissues, it is inducibly expressed during inflammation, oxidative stress, and hyperglycemia. iNOS activity is elevated in a variety of disease states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute kidney injury, TNF-alpha induced muscle wasting, and the increased radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.

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Citations

1. Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns
   Authors: S Altan, Z O?urtan
   Burns, 2017;43(1):232-244.
   Species: Rabbit
   Sample Type: Whole Tissue
   Application: IHC


2. Erythropoietin Receptor Antagonist Suppressed Ectopic Hemoglobin Synthesis in Xenografts of HeLa Cells to Promote Their Destruction.
   Authors: Yasuda Y, Fujita M, Koike E, Obata K, Shiota M, Kotani Y, Musha T, Tsuji-Kawahara S, Satou T, Masuda S, Okano J, Yamasaki H, Okumoto K, Uesugi T, Nakao S, Hoshiai H, Mandai M
   PLoS ONE, 2015;10(4):e0122458.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. High pre-transplant serum nitrate levels predict risk of acute steroid-refractory graft-versus-host disease in the absence of statin therapy.
   Authors: Dietrich S, Okun J, Schmidt K, Falk C, Wagner A, Karamustafa S, Radujkovic A, Hegenbart U, Ho A, Dreger P, Luft T
   Haematologica, 2014;99(3):541-7.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Artichoke, cynarin and cyanidin downregulate the expression of inducible nitric oxide synthase in human coronary smooth muscle cells.
   Authors: Xia N, Pautz A, Wollscheid U, Reifenberg G, Forstermann U, Li H
   Molecules, 2014;19(3):3654-68.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
   BMC Neurosci, 2012;13(0):50.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


6. The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide.
   Authors: Espinosa A, Oke V, Elfving A, Nyberg F, Covacu R, Wahren-Herlenius M
   Exp. Cell Res., 2008;314(20):3605-13.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.
   Authors: Skaleric U, Gaspirc B, McCartney-Francis N, Masera A, Wahl SM
   Infect. Immun., 2006;74(12):7010-3.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Attenuation of apoptosis and enhancement of proteoglycan synthesis in rabbit cartilage defects by hyperbaric oxygen treatment are related to the suppression of nitric oxide production.
   Authors: Yuan LJ, Ueng SW, Lin SS, Yeh WL, Yang CY, Lin PY
   J. Orthop. Res., 2004;22(5):1126-34.
   Species: Rabbit
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded

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