抗Mouse CCL2/JE/MCP-1抗体(Anti-Mouse CCL2/JE/MCP-1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL2/JE/MCP-1Gln24-Arg96Accession # P10148
Source	Monoclonal Rat IgG2B Clone # 123616
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCL2/JE/MCP-1 in ELISAs. Does not cross-react with recombinant human (rh) CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, rmCCL1, 3, 4, 5, 6, 7,  9/10/MIP-1 gamma, 11, 12, 17, 19, 20, 21, 22, 24, 25, 27, 28, or rrCCL20.

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Applications and Data

	Recommended Concentration	Sample
Neutralization	Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.

Neutralization

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL2/JE Monoclonal Antibody (Catalog # MAB479). The ND50 is typically 0.75-3.0 µg/mL.

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Related Product & Information

Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Background	CCL2/JE/MCP-1
background_content	Background: CCL2/JE/MCP-1 Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The mouse CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in mouse fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

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Citations

1. Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids
   Authors: RW Jenkins, AR Aref, PH Lizotte, E Ivanova, S Stinson, CW Zhou, M Bowden, J Deng, H Liu, D Miao, MX He, W Walker, G Zhang, T Tian, C Cheng, Z Wei, S Palakurthi, M Bittinger, H Vitzthum, JW Kim, A Merlino, M Quinn, C Venkataram, JA Kaplan, A Portell, PC Gokhale, B Phillips, A Smart, A Rotem, RE Jones, L Keogh, M Anguiano, L Stapleton, Z Jia, M Barzily-Ro, I Cañadas, TC Thai, MR Hammond, R Vlahos, ES Wang, H Zhang, S Li, GJ Hanna, W Huang, MP Hoang, A Piris, JP Eliane, AO Stemmer-Ra, L Cameron, MJ Su, P Shah, B Izar, M Thakuria, NR LeBoeuf, G Rabinowits, V Gunda, S Parangi, JM Cleary, BC Miller, S Kitajima, R Thummalapa, B Miao, TU Barbie, V Sivathanu, J Wong, WG Richards, R Bueno, CH Yoon, J Miret, M Herlyn, LA Garraway, EM Van Allen, GJ Freeman, PT Kirschmeie, JH Lorch, PA Ott, FS Hodi, KT Flaherty, RD Kamm, GM Boland, KK Wong, D Dornan, CP Paweletz, DA Barbie
   Cancer Discov, 2017;0(0):.
   Species: Human
   Sample Type: organoid
   Application: Neut


2. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
   Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
   Cancer Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


3. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
   Nat Med, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
   Authors: Kowalewska P, Patrick A, Fox-Robichaud A
   PLoS ONE, 2014;9(9):e104537.
   Species: Mouse
   Sample Type: In Vivo
   Application: IHC Fresh


5. Near-infrared laser adjuvant for influenza vaccine.
   Authors: Kashiwagi, Satoshi, Yuan, Jianping, Forbes, Benjamin, Hibert, Mathew L, Lee, Eugene L, Whicher, Laura, Goudie, Calum, Yang, Yuan, Chen, Tao, Edelblute, Beth, Collette, Brian, Edington, Laurel, Trussler, James, Nezivar, Jean, Leblanc, Pierre, Bronson, Roderick, Tsukada, Kosuke, Suematsu, Makoto, Dover, Jeffrey, Brauns, Timothy, Gelfand, Jeffrey, Poznansky, Mark C
   PLoS ONE, 2013;8(12):e82899.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Participation of MyD88 and interleukin-33 as innate drivers of Th2 immunity to Trichinella spiralis.
   Authors: Scalfone, Lisa K, Nel, Hendrik, Gagliardo, Lucille, Cameron, Jody L, Al-Shokri, Shaikha, Leifer, Cynthia, Fallon, Padraic, Appleton, Judith A
   Infect Immun, 2013;81(4):1354-63.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: Neut


7. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
   J. Immunol., 2012;189(5):2545-52.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


8. Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1.
   Authors: Uchida Y, Takeshita K, Yamamoto K, Kikuchi R, Nakayama T, Nomura M, Cheng XW, Egashira K, Matsushita T, Nakamura H, Murohara T
   Diabetes, 2012;61(6):1552-61.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


9. Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
   Authors: Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS
   J. Biol. Chem., 2011;286(23):20991-1001.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


10. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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