抗Mouse CCL5/RANTES抗体(Anti-Mouse CCL5/RANTES antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL5/RANTESSer24-Ser91Accession # P30882
Source	Monoclonal Rat IgG2A Clone # 53405
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCL5/RANTES in direct ELISAs. Shows 50% cross-reactivity with recombinant human (rh) CCL5, and no cross-reactivity with recombinant feline CCL5, rhCCL3, recombinant mouse (rm) CCL3, rmCCL4, rhCCL4, rhCCL14, or rhCCL18.

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Applications and Data

	Recommended Concentration	Sample
Immunocytochemistry	8-25 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.05 µg/mL Recombinant Mouse CCL5/RANTES.

Immunocytochemistry
	CCL5/RANTES in Mouse Splenocytes. CCL5/RANTES was detected in immersion fixed mouse splenocytes using Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Neutralization
	Chemotaxis Induced by CCL5/RANTES and Neutralization by Mouse CCL5/RANTES Antibody. Recombinant Mouse CCL5/RANTES (Catalog # 478-MR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL5/RANTES (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL5/RANTES Monoclonal Antibody (Catalog # MAB478). The ND50 is typically 0.1-0.5 µg/mL.

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Related Product & Information

Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Background	CCL5/RANTES
background_content	Background: CCL5/RANTES CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes (1-3). Human and mouse RANTES exhibit cross-species activity on human and mouse cells (4). Mature mouse CCL5 shares 100% amino acid (aa) sequence identity with rat CCL5 and 75-88% with canine, cotton rat, feline, and human CCL5 (5). CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5 (6, 7). Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28 (8). In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1 (9). The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes (10). Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5’s interaction with the chemokine CXCL4/PF4 (11-13). The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes (14-16). CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of pro-inflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature (17).

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Citations

1. The HIV co-receptor CCR5 regulates osteoclast function
   Authors: JW Lee, A Hoshino, K Inoue, T Saitou, S Uehara, Y Kobayashi, S Ueha, K Matsushima, A Yamaguchi, Y Imai, T Iimura
   Nat Commun, 2017;8(1):2226.
   Species: Moue
   Sample Type: In Vivo
   Application: In vivo


2. CCL5 Promotes Resolution-Phase Macrophage Reprogramming in Concert with the Atypical Chemokine Receptor D6 and Apoptotic Polymorphonuclear Cells
   Authors: M Aswad, S Assi, S Schif-Zuck, A Ariel
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


3. Tick-borne encephalitis virus induces chemokine RANTES expression via activation of IRF-3 pathway
   J Neuroinflammation, 2016;13(1):209.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Tumor-induced pressure in the bone microenvironment causes osteocytes to promote the growth of prostate cancer bone metastases.
   Authors: Sottnik J, Dai J, Zhang H, Campbell B, Keller E
   Cancer Res, 2015;75(11):2151-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Rapid dendritic cell mobilization to the large intestinal epithelium is associated with resistance to Trichuris muris infection.
   Authors: Cruickshank SM, Deschoolmeester ML, Svensson M, Howell G, Bazakou A, Logunova L, Little MC, English N, Mack M, Grencis RK, Else KJ, Carding SR
   J. Immunol., 2009;182(5):3055-62.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes.
   Authors: Herz J, Pardo J, Kashkar H, Schramm M, Kuzmenkina E, Bos E, Wiegmann K, Wallich R, Peters PJ, Herzig S, Schmelzer E, Kronke M, Simon MM, Utermohlen O
   Nat. Immunol., 2009;10(7):761-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


7. Joint capsule treatment with enkephalin-encoding HSV-1 recombinant vector reduces inflammatory damage and behavioural sequelae in rat CFA monoarthritis.
   Authors: Lu Y, McNearney TA, Wilson SP, Yeomans DC, Westlund KN
   Eur. J. Neurosci., 2008;27(5):1153-65.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
   Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
   J. Exp. Med., 2008;205(9):2075-84.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
   Authors: Fujita H, Asahina A, Komine M, Tamaki K
   Cell. Immunol., 2007;245(2):70-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


10. Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6.
    Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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