抗Mouse CCL3/MIP-1 alpha抗体(Anti-Mouse CCL3/MIP-1 alpha antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92Accession # P10855
Source	Monoclonal Rat IgG2A Clone # 39624
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCL3/MIP‑1 alpha in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse CCL4/MIP‑1 beta  is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 21, 22, 23, 24, 25, 28, recombinant mouse CCL1, 2, 6, 7, 9/10/MIP-1 gamma, 11, 12, 19, 21, 22, 25, 28, recombinant rat CCL2 or CCL20 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse CCL3/MIP-1 alpha Isoform LD78a (Catalog # 450-MA) under non-reducing conditions only

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Related Product & Information

Background

CCL3/MIP-1 alpha

background_content

Background: CCL3/MIP-1 alpha The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse CCL3/MIP-1 alpha shares approximately 77% and 70% amino acid identity with human CCL3/MIP-1 alpha and mouse CCL4/MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP‑1 proteins are members of the beta  (C‑C) subfamily of chemokines. Both CCL3 and CCL4 are monocyte chemoattractants in vitro. Additionally, the MIP‑1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with CCL3 and CCL4 preferentially attracting CD8+ and CD4+ T cells, respectively. CCL3 has also been shown to attract B cells as well as eosinophils. MIP‑1 proteins have been reported to have multiple effects on hematopoietic precursor cells, and CCL3 has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, CCL4 was reported to be much less active. The functional receptor for CCL3 has been identified as CCR1 and CCR5.

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Citations

1. Immune signatures of protective spleen memory CD8 T cells
   Sci Rep, 2016;6(0):37651.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Mechanisms of Invariant NKT Cell Activity in Restraining Bacillus anthracis Systemic Dissemination
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. Induction of the MCP chemokine cluster cascade in the periphery by cancer cell-derived CCL3
   Authors: Elena Farmaki
   Cancer Lett, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: Neut


4. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
   Authors: Deepak P, Kumar S, Kishore D, Acharya A
   Int. Immunol., 2010;22(1):53-63.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
   Authors: Fujita H, Asahina A, Komine M, Tamaki K
   Cell. Immunol., 2007;245(2):70-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


7. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
   Authors: Mosmann T
   Eur. J. Immunol., 2004;34(6):1617-26.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
   Authors: Yang L, Mosmann T
   Eur. J. Immunol., 2004;34(6):1617-26.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. A soluble chemokine-binding protein from vaccinia virus reduces virus virulence and the inflammatory response to infection.
   Authors: Reading PC, Symons JA, Smith GL
   J. Immunol., 2003;170(3):1435-42.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


10. Regulatory effects of eotaxin on acute lung inflammatory injury.
    Authors: Guo RF, Lentsch AB, Warner RL, Huber-Lang M, Sarma JV, Hlaing T, Shi MM, Lukacs NW, Ward PA
    J. Immunol., 2001;166(8):5208-18.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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