抗SSEA-4抗体(Anti-SSEA-4, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	2120Ep human embryonal carcinoma cell line
Source	Monoclonal Mouse IgG3 Clone # MC-813-70
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Recognizes a carbohydrate epitope of SSEA-4 (1, 2).

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry. NTera‑2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA‑4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
	SSEA‑4 in BG01V Human Stem Cells. SSEA‑4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA‑4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

SSEA-4

background_content

Background: SSEA-4 SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

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Citations

1. A Comprehensive TALEN-Based Knockout Library for Generating Human Induced Pluripotent Stem Cell-Based Models for Cardiovascular Diseases
   Authors: I Karakikes, V Termglinch, DA Cepeda, J Lee, S Diecke, A Hendel, I Itzhaki, M Ameen, R Shrestha, H Wu, N Ma, NY Shao, T Seeger, NA Woo, KD Wilson, E Matsa, MH Porteus, V Sebastiano, JC Wu
   Circ. Res, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene
   Authors: A Khudiakov, D Kostina, A Zlotina, T Nikulina, A Sergushich, A Gudkova, A Tomilin, A Malashiche, A Kostareva
   Stem Cell Res, 2017;24(0):77-80.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
   Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
   Sci Rep, 2017;7(1):10750.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


4. Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts
   Authors: SI Pao, KH Chien, HT Lin, MC Tai, JT Chen, CM Liang
   J Chin Med Assoc, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
   Authors: NG Kooreman, PE de Almeida, JP Stack, RV Nelakanti, S Diecke, NY Shao, RJ Swijnenbur, V Sanchez-Fr, E Matsa, C Liu, AJ Connolly, JF Hamming, PHA Quax, MA Brehm, DL Greiner, LD Shultz, JC Wu
   Cell Rep, 2017;20(8):1978-1990.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. The effects of glomerular and tubular renal progenitors and derived extracellular vesicles on recovery from acute kidney injury
   Authors: A Ranghino, S Bruno, B Bussolati, A Moggio, V Dimuccio, M Tapparo, L Biancone, P Gontero, B Frea, G Camussi
   Stem Cell Res Ther, 2017;8(1):24.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
   Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
   Stem Cell Res, 2017;20(0):105-114.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Modeling Glanzmann thrombasthenia using patient specific iPSCs and restoring platelet aggregation function by CD41 overexpression
   Authors: L Hu, L Du, Y Zhao, W Li, Q Ouyang, D Zhou, G Lu, G Lin
   Stem Cell Res, 2017;20(0):14-20.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. A Novel Molecular and Functional Stemness Signature Assessing Human Cord Blood-Derived Endothelial Progenitor Cell Immaturity
   Authors: O Guillevic, S Ferratge, J Pascaud, C Driancourt, J Boyer-Di-P, G Uzan
   PLoS ONE, 2016;11(4):e0152993.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


10. Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells.
    Authors: Zeng S, Liu L, Sun Y, Xie P, Hu L, Yuan D, Chen D, Ouyang Q, Lin G, Lu G
    J Cell Sci, 2014;127(0):752-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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