抗SSEA-3抗体(Anti-SSEA-3, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Four to eight cell stage mouse embryos
Source	Monoclonal Rat IgM Clone # MC-631
Purification	IgM-specific Affinity-purified from hybridoma culture supernatant
Specificity	Recognizes a carbohydrate epitope of SSEA-3 (4, 5).

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	D3 mouse embryonic stem cell line
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

SSEA‑3 in BG01V Human Stem Cells. SSEA‑3 was detected in immersion fixed non-permeabilized BG01V human embryonic stem cells using Human/Mouse SSEA‑3 Monoclonal Antibody (Catalog # MAB1434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

SSEA-3

background_content

Background: SSEA-3 SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

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Citations

1. Progesterone Receptor Membrane Component 1 suppresses the p53 and Wnt/?-catenin pathways to promote human pluripotent stem cell self-renewal
   Authors: JY Kim, SY Kim, HS Choi, MK Kim, HM Lee, YJ Jang, CJ Ryu
   Sci Rep, 2018;8(1):3048.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
   Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
   Stem Cell Res, 2017;20(0):105-114.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Induction of Pluripotent Stem Cells from a Manifesting Carrier of Duchenne Muscular Dystrophy and Characterization of Their X-Inactivation Status
   Authors: Y Miyagoe-Su, T Nishiyama, M Nakamura, A Narita, F Takemura, S Masuda, N Minami, K Murayama, H Komaki, YI Goto, S Takeda
   Stem Cells Int, 2017;2017(0):7906843.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


4. High expression FUT1 and B3GALT5 is an independent predictor of postoperative recurrence and survival in hepatocellular carcinoma
   Authors: HH Kuo, RJ Lin, JT Hung, CB Hsieh, TH Hung, FY Lo, MY Ho, CT Yeh, YL Huang, J Yu, AL Yu
   Sci Rep, 2017;7(1):10750.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


5. A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.
   Authors: Matsumoto S, Nakao H, Kawabe K, Nonaka M, Toyoda H, Takishima Y, Kawabata K, Yamaguchi T, Furue M, Taki T, Okumura T, Yamazaki Y, Nakaya S, Kawasaki N, Kawasaki T
   J Biol Chem, 2016;290(33):20071-85.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - PFA fixed


6. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
   Sci Rep, 2016;6(0):31063.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC-Fluorescent


7. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
   Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
   Stem Cells, 2014;32(10):2626-41.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
   BMC Vet Res, 2012;8(0):150.
   Species: Canine
   Sample Type: Whole Cells
   Application: Flow


9. Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells.
   Authors: Ware CB, Wang L, Mecham BH, Shen L, Nelson AM, Bar M, Lamba DA, Dauphin DS, Buckingham B, Askari B, Lim R, Tewari M, Gartler SM, Issa JP, Pavlidis P, Duan Z, Blau CA
   Cell Stem Cell, 2009;4(4):359-69.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


10. Secreted proteoglycans directly mediate human embryonic stem cell-basic fibroblast growth factor 2 interactions critical for proliferation.
    Authors: Levenstein ME, Berggren WT, Lee JE, Conard KR, Llanas RA, Wagner RJ, Smith LM, Thomson JA
    Stem Cells, 2008;26(12):3099-107.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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