抗Mouse IL-17抗体(Anti-Mouse IL-17 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL‑17Thr22-Ala158Accession # Q62386
Source	Monoclonal Rat IgG2A Clone # 50104
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-17 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% reactivity with recombinant mouse(rm) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant human IL-17, recombinant canine IL-17, rmIL-17B,rmIL‑17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.

Western Blot
	Detection of Recombinant Mouse IL‑17/IL‑17A by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL‑17/IL‑17A (Catalog # 421-ML), Recombinant Human IL‑17/IL‑17A (Catalog # 317-ILB), Recombinant Rat IL‑17/IL‑17A (Catalog # 8410-IL), and Recombinant Mouse IL‑17F (Catalog # 2057-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL‑17/IL‑17A Monoclonal Antibody (Catalog # MAB421) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL‑17/IL‑17A at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	IL‑6 Secretion Induced byIL‑17 and Neutralization by Mouse IL‑17 Antibody. Recombinant Mouse IL‑17 (Catalog # 421-ML) stimulates IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Mouse IL‑17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑17 Monoclonal Antibody (Catalog # MAB421). The ND50 is typically0.05-0.15 µg/mL.

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Related Product & Information

Long Name	Interleukin 17
Entrez Gene IDs	3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
Background	IL-17/IL-17A
background_content	Background: IL-17/IL-17A Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mIL-17 shows 57% and 87% sequence identity with herpesvirus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR+CD4-CD8-T cells, the expression of mIL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

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Citations

1. Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer
   Authors: AP Gounder, CC Yokoyama, NN Jarjour, TL Bricker, BT Edelson, ACM Boon
   PLoS Pathog., 2018;14(4):e1007001.
   Species: Mouse
   Sample Type: BALF
   Application: WB


2. Estrogen Deficiency Promotes Cerebral Aneurysm Rupture by Upregulation of Th17 Cells and Interleukin-17A Which Downregulates E-Cadherin
   Authors: BL Hoh, K Rojas, L Lin, HZ Fazal, S Hourani, KW Nowicki, MB Schneider, K Hosaka
   J Am Heart Assoc, 2018;7(8):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis
   Authors: CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho
   Cell Death Dis, 2018;9(3):401.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


4. IL-17A Produced by Innate Lymphoid Cells Is Essential for Intestinal Ischemia-Reperfusion Injury
   Authors: M Geha, MG Tsokos, RE Bosse, T Sannikova, Y Iwakura, JJ Dalle Lucc, R De Waal Ma, GC Tsokos
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


5. Galectin-1-Driven Tolerogenic Programs Aggravate Yersinia enterocolitica Infection by Repressing Antibacterial Immunity
   Authors: RC Davicino, SP Méndez-Hue, RJ Eliçabe, JC Stupirski, I Autenrieth, MS Di Genaro, GA Rabinovich
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


6. IL-35 induces N2 phenotype of neutrophils to promote tumor growth
   Authors: JM Zou, J Qin, YC Li, Y Wang, D Li, Y Shu, C Luo, SS Wang, G Chi, F Guo, GM Zhang, ZH Feng
   Oncotarget, 2017;8(20):33501-33514.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


7. Spirulina lipopolysaccharides inhibit tumor growth in a Toll-like receptor 4-dependent manner by altering the cytokine milieu from interleukin-17/interleukin-23 to interferon-?
   Authors: H Okuyama, A Tominaga, S Fukuoka, T Taguchi, Y Kusumoto, S Ono
   Oncol. Rep, 2017;37(2):684-694.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. IL-17A exacerbates diabetic retinopathy by impairing M�ller cell function via Act1 signaling
   Authors: Ao-Wang Qiu
   Exp. Mol. Med, 2016;48(12):e280.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


9. IL-17 Augments B Cell Activation in Ocular Surface Autoimmunity
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


10. Seminal plasma induces inflammation in the uterus through the ?? T/IL-17 pathway
    Authors: ZH Song, ZY Li, DD Li, WN Fang, HY Liu, DD Yang, CY Meng, Y Yang, JP Peng
    Sci Rep, 2016;6(0):25118.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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