抗Glucose Transporter 1抗体(Anti-Glucose Transporter 1, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NS0 mouse myeloma cell line transfected with human Glut1Met1-Val492Accession # AAA52571
Source	Monoclonal Mouse IgG2B Clone # 202915
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Flow Cytometry
	Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Flow Cytometry
	Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
	Glut1 in HepG2 Human Hepatocellular Carcinoma Cell Line. Glut1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB1418) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background

Glut1

background_content

Background: Glut1 Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

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Citations

1. Manipulating Glucose Metabolism during Different Stages of Viral Pathogenesis Can Have either Detrimental or Beneficial Effects
   Authors: SK Varanasi, D Donohoe, U Jaggi, BT Rouse
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Emerging Role and Characterization of Immunometabolism: Relevance to HIV Pathogenesis, Serious Non-AIDS Events, and a Cure
   J Immunol, 2016;196(11):4437-44.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Foxp3-mediated inhibition of Akt inhibits Glut1 (glucose transporter 1) expression in human T regulatory cells.
   Authors: Basu S, Hubbard B, Shevach E
   J Leukoc Biol, 2015;97(2):279-83.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Increased glucose metabolic activity is associated with CD4+ T-cell activation and depletion during chronic HIV infection.
   Authors: Palmer C, Ostrowski M, Gouillou M, Tsai L, Yu D, Zhou J, Henstridge D, Maisa A, Hearps A, Lewin S, Landay A, Jaworowski A, McCune J, Crowe S
   AIDS, 2014;28(3):297-309.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. The chemokine CCL5 regulates glucose uptake and AMP kinase signaling in activated T cells to facilitate chemotaxis.
   Authors: Chan O, Burke J, Gao D, Fish E
   J Biol Chem, 2012;287(35):29406-16.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Entry of human T-cell leukemia virus type 1 is augmented by heparin sulfate proteoglycans bearing short heparin-like structures.
   Authors: Tanaka A, Jinno-Oue A, Shimizu N
   J. Virol., 2012;86(6):2959-69.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
   Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
   J. Virol., 2009;83(21):10908-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Antiparallel segregation of notch components in the immunological synapse directs reciprocal signaling in allogeneic Th:DC conjugates.
   Authors: Luty WH, Rodeberg D, Parness J, Vyas YM
   J. Immunol., 2007;179(2):819-29.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. Role of FDG-PET as a biological marker for predicting the hypoxic status of tongue cancer.
   Authors: Han M, Lee H, Cho K, Kim J, Roh J, Choi S, Nam S, Kim S
   Head Neck, 0;34(10):1395-402.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC

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