抗Human CX3CL1/Fractalkine抗体(Anti-Human CX3CL1/Fractalkine antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human CX3CL1/Fractalkine Chemokine Domain Gln25-Arg339 (Ser199Asn) (predicted)Accession # P78423
Source	Monoclonal Mouse IgG1 Clone # 51637
Purification	Protein A or G purified from ascites
Specificity	Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse CX3CL1 or recombinant rat CX3CL1 is observed.

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Applications and Data

	Recommended Concentration	Sample
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	Human CX3CL1/Fractalkine transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin

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Related Product & Information

Entrez Gene IDs	6376 (Human); 20312 (Mouse); 89808 (Rat)
Background	CX3CL1/Fractalkine
background_content	Background: CX3CL1/Fractalkine CX3CL1, also named neurotactin, is a novel chemokine identified through bioinformatics. CX3CL1 has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, CX3CL1 is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human CX3CL1 cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human CX3CL1 can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble CX3CL1. CX3CL1 mRNA has been detected in various tissues including the brain and heart. The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes.

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Citations

1. Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion
   Authors: A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig
   Mediators Inflamm., 2017;2017(0):1515389.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


2. Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity.
   Authors: Suzuki F, Kubota T
   Arthritis Res. Ther., 2012;14(2):R48.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


3. CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis.
   Authors: Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD
   Blood, 2008;112(13):5026-36.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
   Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
   J. Immunol., 2007;178(2):1172-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Cell-based ELISA


5. The uremic solute p-cresol decreases leukocyte transendothelial migration in vitro.
   Authors: Faure V, Cerini C, Paul P, Berland Y, Dignat-George F, Brunet P
   Int. Immunol., 2006;18(10):1453-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation.
   Authors: Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M
   Eur. J. Neurosci., 2004;20(12):3222-32.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth.
   Authors: Chazaud B, Sonnet C, Lafuste P, Bassez G, Rimaniol AC, Poron F, Authier FJ, Dreyfus PA, Gherardi RK
   J. Cell Biol., 2003;163(5):1133-43.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. The transmembrane form of the CX3CL1 chemokine fractalkine is expressed predominantly by epithelial cells in vivo.
   Authors: Lucas AD, Chadwick N, Warren BF, Jewell DP, Gordon S, Powrie F, Greaves DR
   Am. J. Pathol., 2001;158(3):855-66.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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