抗Human CXCR1/IL-8 RA抗体(Anti-Human CXCR1/IL-8 RA antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NS0 mouse myeloma cell line transfected with human CXCR1/IL-8 RAMet1-Leu350Accession # AAA59159
Source	Monoclonal Mouse IgG2A Clone # 42705
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human CXCR1/IL-8 RA transfectants but not the parental cell line. It does not cross-react with human CXCR2.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-reported	Cheng, Y. et al. (2016) J. Immunol. 196: 924. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR1. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 1 ng/mL Recombinant Human CXCL8/IL‑8.

Flow Cytometry
	Detection of CXCR1/IL‑8 RA in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-HumanCXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Neutralization
	Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Human CXCR1/IL‑8 RA Antibody. Recombinant Human CXCL8/IL‑8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL8/IL‑8 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR1/IL‑8 RA Monoclonal Antibody (Catalog # MAB330). The ND50 is typically 0.4‑2.0 µg/mL.

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Related Product & Information

Long Name	Interleukin 8 Receptor A
Entrez Gene IDs	3577 (Human); 227288 (Mouse); 54258 (Rat)
Background	CXCR1/IL-8 RA
background_content	Background: CXCR1/IL-8 RA The human C-X-C chemokine IL-8 is a potent neutrophil chemotactic and activating factor. Two distinct G protein-linked cell surface receptors, known as IL-8 RA (type I or CXCR1) and IL-8 RB (type II or CXCR2), can interact with the IL-8 molecule. These two receptors share 77% amino acid homology. CXCR1 expression has been documented on neutrophils, monocytes, and a small population of T cells.

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Citations

1. Altered monocyte differentiation and macrophage polarization patterns in patients with breast cancer
   Authors: CH Hung, FM Chen, YC Lin, ML Tsai, SL Wang, YC Chen, YT Chen, MF Hou
   BMC Cancer, 2018;18(1):366.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Role of autophagy in the HIV-1 matrix protein p17-driven lymphangiogenesis
   Authors: P Mazzuca, S Marsico, K Schulze, S Mitola, MC Pils, C Giagulli, CA Guzman, A Caruso, F Caccuri
   J. Virol., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients
   Authors: ALT Loi, S Hoonhorst, C van Aalst, J Langereis, V Kamp, S Sluis-Eisi, N Ten Hacken, JW Lammers, L Koenderman
   Respir. Res., 2017;18(1):100.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs
   Authors: Nader Alaridah
   J Innate Immun, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


5. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines.
   Authors: Al-Alwan L, Chang Y, Rousseau S, Martin J, Eidelman D, Hamid Q
   J Immunol, 2015;193(3):1416-26.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. The endothelin-integrin axis is involved in macrophage-induced breast cancer cell chemotactic interactions with endothelial cells.
   Authors: Chen C, Chen L, Hsu Y, Liu K, Fan C, Huang T
   J Biol Chem, 2014;289(14):10029-44.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
   Authors: Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H
   Eur J Immunol, 2014;44(2):420-30.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
   Authors: Caccuri, Francesc, Giagulli, Cinzia, Reichelt, Joachim, Martorelli, Debora, Marsico, Stefania, Bugatti, Antonell, Barone, Ines, Rusnati, Marco, Guzman, Carlos A, Dolcetti, Riccardo, Caruso, Arnaldo
   J Virol, 2014;88(10):5706-17.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. The IL-8/CXCR1 axis is associated with cancer stem cell-like properties and correlates with clinical prognosis in human pancreatic cancer cases.
   Authors: Chen, Lianyu, Fan, Jie, Chen, Hao, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
   Sci Rep, 2014;4(0):5911.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
    Authors: Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J
    Cytokine, 2014;67(2):53-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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