抗Oligodendrocyte Marker O4抗体(Anti-Oligodendrocyte Marker O4, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat, Chicken
Label	Unconjugated
Immunogen	Bovine brain corpus callosum white matter
Source	Monoclonal Mouse IgM Clone # O4
Purification	IgM-specific Affinity-purified from hybridoma culture supernatant
Specificity	Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	1-10 µg/mL	See below

Flow Cytometry
	Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells (Catalog # NSC001) either differentiated (filled histogram) or undifferentiated (open histogram) were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326), followed by PE-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
	Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed 7 day differentiated rat cortical stem cells using 1 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Background	Oligodendrocyte Marker O4
background_content	Background: Oligodendrocyte Marker O4 Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

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Citations

1. INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice
   Authors: O Le, L Palacio, G Bernier, I Batinic-Ha, G Hickson, C Beauséjour
   Stem Cell Reports, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. Comprehensive integrative analyses identify GLT8D1 and CSNK2B as schizophrenia risk genes
   Authors: CP Yang, X Li, Y Wu, Q Shen, Y Zeng, Q Xiong, M Wei, C Chen, J Liu, Y Huo, K Li, G Xue, YG Yao, C Zhang, M Li, Y Chen, XJ Luo
   Nat Commun, 2018;9(1):838.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Protein-Induced Pluripotent Stem Cells Ameliorate Cognitive Dysfunction and Reduce A? Deposition in a Mouse Model of Alzheimer's Disease
   Authors: MY Cha, YW Kwon, HS Ahn, H Jeong, YY Lee, M Moon, SH Baik, DK Kim, H Song, EC Yi, D Hwang, HS Kim, I Mook-Jung
   Stem Cells Transl Med, 2017;6(1):293-305.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


4. Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain
   Authors: A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller
   Neuron, 2017;94(3):500-516.e9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Unique spectral signatures of the nucleic acid dye acridine orange can distinguish cell death by apoptosis and necroptosis
   Authors: JR Plemel, AV Caprariell, MB Keough, TJ Henry, S Tsutsui, TH Chu, GJ Schenk, R Klaver, VW Yong, PK Stys
   J. Cell Biol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro
   Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
   Mol Med Rep, 2017;0(0):.
   Species: Rat
   Sample Type: Whole Cells
   Application: ICC


7. Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency
   Authors: JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia
   Cell Rep, 2017;19(1):20-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. BDE-99 impairs differentiation of human and mouse NPCs into the oligodendroglial lineage by species-specific modes of action
   Authors: K Dach, F Bendt, U Huebenthal, S Giersiefer, PJ Lein, H Heuer, E Fritsche
   Sci Rep, 2017;7(0):44861.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


9. Tumour-associated glial host cells display a stem-like phenotype with a distinct gene expression profile and promote growth of GBM xenografts
   Authors: L Leiss, E Mutlu, A Øyan, T Yan, O Tsinkalovs, L Sleire, K Petersen, MA Rahman, M Johannesse, SS Mitra, HK Jacobsen, KM Talasila, H Miletic, I Jonassen, X Li, NH Brons, KH Kalland, J Wang, PØ Enger
   BMC Cancer, 2017;17(1):108.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


10. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells
    Authors: T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato
    PLoS ONE, 2017;12(2):e0171947.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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