抗Human HGF抗体(Anti-Human HGF antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGFGln32-Ser728Accession # P14210
Source	Monoclonal Mouse IgG1 Clone # 24612
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human HGF in Western blots. In Western blots, no-cross reactivity with recombinant mouse HGF or recombinant canine HGF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Neutralization	Measured by its ability to neutralize HGF-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 100 ng/mL Recombinant Human HGF.

Western Blot
	Detection of Recombinant Human, Mouse, and Canine HGF by Western Blot. Western blot shows 25 ng of Recombinant Human HGF (Catalog # 294-HG), Recombinant Mouse HGF (Catalog # 2207-HG) and Recombinant Canine HGF (Catalog # 3386-HG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HGF at approximately 75 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	Cell Proliferation Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF (Catalog # 294-HG) stimulates proliferation in the 4MBr‑5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HGF (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294). The ND50 is typically0.1-0.3 µg/mL.

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Related Product & Information

Long Name	Hepatocyte Growth Factor
Entrez Gene IDs	3082 (Human); 15234 (Mouse); 403441 (Canine)
Background	HGF
background_content	Background: HGF HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.

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Citations

1. Hepatocyte growth factor and p38 promote osteogenic differentiation of human mesenchymal stem cells.
   Authors: Aenlle K, Curtis K, Roos B, Howard G
   Mol Endocrinol, 2014;28(5):722-30.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
   Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
   Cell Stem Cell, 2014;14(3):342-56.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


3. Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
   Authors: Tyan SW, Kuo WH, Huang CK
   PLoS ONE, 2011;6(1):e15313.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. A multiplex immunoassay for human adipokine profiling.
   Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
   Clin. Chem., 2010;56(0):1320.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


5. Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models.
   Authors: Coxon A, Rex K, Meyer S, Sun J, Sun J, Chen Q, Radinsky R, Kendall R, Burgess TL
   Mol. Cancer Ther., 2009;0(0):.
   Species: Human
   Sample Type: Recombinant Protein
   Application: Neut


6. alpha vbeta 6 Integrin promotes the invasion of morphoeic basal cell carcinoma through stromal modulation.
   Authors: Marsh D, Dickinson S, Neill GW, Marshall JF, Hart IR, Thomas GJ
   Cancer Res., 2008;68(9):3295-303.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
   Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
   Int. J. Cancer, 2006;119(10):2313-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
   Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
   J. Immunol., 2005;175(9):6177-89.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Neut


9. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
   Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
   Cancer Res., 2003;63(4):887-92.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts.
    Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M
    Transplant. Proc., 2000;32(5):987-9.
    Species: Porcine
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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