抗Human CCL2/MCP-1抗体(Anti-Human CCL2/MCP-1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human CCL2/JE/MCP-1
Source	Monoclonal Mouse IgG1 Clone # 24822
Purification	Protein A or G purified from ascites
Specificity	Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4, 5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5, 6, 7, 9/10MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.9-4.5 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP‑1.

Western Blot
	Detection of Recombinant Human CCL2/JE/MCP‑1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP‑1 (Catalog # 279-MC), Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP), Recombinant Human CCL7/MCP‑3/MARC (Catalog # 282-P3), Recombinant Human CCL13/MCP‑4 (Catalog # 327-P4), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Mouse CCL2/JE/MCP‑1 (Catalog # 479-JE), and Recombinant Rat CCL2/JE/MCP‑1 (Catalog # 3144-JE). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP‑1 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Neutralization
	Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Human CCL2/JE/MCP‑1 Antibody. Recombinant Human CCL2/JE/MCP‑1 (Catalog #279-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/JE/MCP‑1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations ofMouse Anti-Human CCL2/JE/MCP‑1 Monoclonal Antibody (Catalog # MAB279). The ND50 is typically 0.9-4.5 µg/mL.

目次に戻る

Related Product & Information

Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
Background	CCL2/JE/MCP-1
background_content	Background: CCL2/JE/MCP-1 CCL2 is a beta -chemokine that is also known as monocyte chemotactic protein 1 (MCP-1). It is produced by a variety of cell types and is chemotactic for monocytes.

目次に戻る

Citations

1. Continuous Delivery of Neutralizing Antibodies Elevate CCL2 Levels in Mice Bearing MCF10CA1d Breast Tumor Xenografts
   Authors: M Yao, C Smart, Q Hu, N Cheng
   Transl Oncol, 2017;10(5):734-743.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


2. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
   Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
   J Neurosci, 2015;35(6):2452-64.
   Species: Rat
   Sample Type: Whole Cells
   Application: Neut


3. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
   J. Exp. Med., 2012;209(10):1813-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
   Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
   Int. J. Cancer, 2010;126(5):1095-108.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
   Authors: Renaud SJ, Sullivan R, Graham CH
   Placenta, 2009;30(4):313-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua.
   Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ
   J. Pathol., 2008;214(3):328-36.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: ELISA Development


7. Induction of host chemotactic response by Encephalitozoon spp.
   Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H
   Infect. Immun., 2007;75(4):1619-25.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
   Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
   Atherosclerosis, 2007;192(1):56-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
   Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
   Clin. Cancer Res., 2007;13(19):5719-28.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: ELISA Development


10. Systemic inflammation in nonalcoholic fatty liver disease is characterized by elevated levels of CCL2.
    Authors: Haukeland JW, Damas JK, Konopski Z, Loberg EM, Haaland T, Goverud I, Torjesen PA, Birkeland K, Bjoro K, Aukrust P
    J. Hepatol., 2006;44(6):1167-74.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

目次に戻る