抗DC-SIGN2抗体(Anti-DC-SIGN2, Human, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299Accession # Q9H2X3
Source	Monoclonal Mouse IgG2B Clone # 120604
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human DC‑SIGNR/CD299.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/106 cells	See below
Adhesion Blockade	The adhesion of NIH-3T3 mouse embryonic fibroblast cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-3/CD50 Fc Chimera (Catalog # 715-IC, 5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 2.5 µg/mL of the antibody.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

Detection of DC‑SIGNR/CD299 in Human DC‑SIGNR/CD299 Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC‑SIGNR/CD299 and DC‑SIGN transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC‑SIGNR/CD299 Monoclonal Antibody (Catalog # MAB162, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).

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Related Product & Information

Background

DC-SIGNR/CD299

background_content

Background: DC-SIGNR/CD299 Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

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Citations

1. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
   Authors: Johnson TR, McLellan JS, Graham BS
   J. Virol., 2012;86(3):1339-47.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
   Authors: Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
   J. Hepatol., 2009;51(4):734-49.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
   Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
   J. Virol., 2009;83(21):10908-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. DC-SIGN and CLEC-2 mediate human immunodeficiency virus type 1 capture by platelets.
   Authors: Chaipan C, Soilleux EJ, Simpson P, Hofmann H, Gramberg T, Marzi A, Geier M, Stewart EA, Eisemann J, Steinkasserer A, Suzuki-Inoue K, Fuller GL, Pearce AC, Watson SP, Hoxie JA, Baribaud F, Pohlmann S
   J. Virol., 2006;80(18):8951-60.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
   Authors: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH
   Am. J. Pathol., 2006;169(1):200-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


6. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
   Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
   Gut, 2006;56(2):243-52.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Hepatitis C virus targets DC-SIGN and L-SIGN to escape lysosomal degradation.
   Authors: Ludwig IS, Lekkerkerker AN, Depla E, Bosman F, Musters RJ, Depraetere S, van Kooyk Y, Geijtenbeek TB
   J. Virol., 2004;78(15):8322-32.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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