抗Human Decorin抗体(Anti-Human Decorin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DecorinGly17-Lys172Accession # NP_598013.1
Source	Monoclonal Mouse IgG1 Clone # 115402
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human Decorin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse Decorin.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of human breast

Western Blot

Detection of Human Decorin by Western Blot. Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Background

Decorin

background_content

Background: Decorin Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR) (1, 2). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. The 329 aa mature protein contains twelve LRR. Alternate splicing generates five isoforms with variable length deletions (3). Mature human and mouse Decorin share 80% aa sequence identity. In Decorin, serine 34 in the N-terminal domain is O-glycosylated. Naturally occurring Decorin proteoglycan has a molecular mass of approximately 100 kDa, and the deglycosylated Decorin core protein has a mass of approximately 40 kDa. Decorin binds to fibronectin, TGF-beta, and type I and type II collagens. The binding of Decorin to various molecules was reported to be mediated via the core protein. Decorin has been implicated in matrix assembly and has also been reported to suppress the growth of various tumor cell lines by activating the epidermal growth factor receptor.

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Citations

1. Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome
   Authors: N Chiarelli, G Carini, N Zoppi, M Ritelli, M Colombi
   PLoS ONE, 2018;13(1):e0191220.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


2. Dermatan sulfate is a player in the transglutaminase 2 interaction network
   Authors: G Wisowski, EM Ko?ma, T Bielecki, A Pude?ko, K Olczyk
   PLoS ONE, 2017;12(2):e0172263.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: IP


3. Proteomics of thyroid tumours provides new insights into their molecular composition and changes associated with malignancy
   Authors: J Martínez-A, R Clifton-Bl, MP Molloy
   Sci Rep, 2016;6(0):23660.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


4. Possible dual role of decorin in abdominal aortic aneurysm.
   Authors: Ueda, Koshiro, Yoshimura, Koichi, Yamashita, Osamu, Harada, Takasuke, Morikage, Noriyasu, Hamano, Kimikazu
   PLoS ONE, 2015;10(3):e0120689.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


5. Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta.
   Authors: Syx D, Guillemyn B, Symoens S, Sousa A, Medeira A, Whiteford M, Hermanns-Le T, Coucke P, De Paepe A, Malfait F
   J Bone Miner Res, 2015;30(8):1445-56.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


6. Faulty initiation of proteoglycan synthesis causes cardiac and joint defects.
   Authors: Baasanjav S, Al-Gazali L, Hashiguchi T, Mizumoto S, Fischer B, Horn D, Seelow D, Ali BR, Aziz SA, Langer R, Saleh AA, Becker C, Nurnberg G, Cantagrel V, Gleeson JG, Gomez D, Michel JB, Stricker S, Lindner TH, Nurnberg P, Sugahara K, Mundlos S, Hoffmann K
   Am. J. Hum. Genet., 2011;89(1):15-27.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


7. Lack of CXC chemokine receptor 3 signaling leads to hypertrophic and hypercellular scarring.
   Authors: Yates CC, Krishna P, Whaley D, Bodnar R, Turner T, Wells A
   Am. J. Pathol., 2010;176(4):1743-55.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Role of decorin in the antimyeloma effects of osteoblasts.
   Authors: Li X, Pennisi A, Yaccoby S
   Blood, 2008;112(1):159-68.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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