抗Human/Mouse SSEA-4 Phycoerythrin抗体(Anti-Human/Mouse SSEA-4 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human, Mouse
Label	Phycoerythrin
Immunogen	2120Ep human embryonal carcinoma cell line
Source	Monoclonal Mouse IgG3 Clone # MC-813-70
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Recognizes a carbohydrate epitope of SSEA-4 (1, 2).

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 PE-conjugated Mono­clonal Antibody (Catalog # FAB1435P, filled histogram) or isotype control antibody (Catalog # IC007P, open histogram). View our protocol for Staining Membrane-associated Proteins.

目次に戻る

Related Product & Information

Background

SSEA-4

background_content

Background: SSEA-4 SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

目次に戻る

Citations

1. A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling
   Authors: S Klein, JL Dvornik, AR Yarrabothu, C Schaniel
   Stem Cell Res, 2017;23(0):73-76.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. An alternative pluripotent state confers interspecies chimaeric competency.
   Authors: Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J
   Nature, 2015;521(7552):316-21.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
   Stem Cells, 2012;29(9):1469-74.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
   Gene Ther, 2012;19(10):1035-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.
   Authors: Fekete N, Rojewski M, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H
   PLoS ONE, 2012;7(8):e43255.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Human dermis harbors distinct mesenchymal stromal cell subsets.
   Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
   J. Invest. Dermatol., 2012;132(3):563-74.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
   Authors: Carvajal-Vergara X, Sevilla A, D'Souza SL
   Nature, 2010;465(7299):808-12.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
   Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
   Cancer Res., 2009;69(14):5648-55.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


9. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery.
   Authors: Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG, Andersen CY, Satir P, Bouhassira EE, Christensen ST, Hirsch RE
   J. Cell Biol., 2008;180(5):897-904.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. T lineage differentiation from human embryonic stem cells.
    Authors: Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11742-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

目次に戻る