抗TREM-1抗体(Anti-TREM-1, Human, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1Ala21-Asn205Accession # Q9NP99
Source	Monoclonal Mouse IgG1 Clone # 193015
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human TREM-1 in ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Background

TREM-1

background_content

Background: TREM-1 TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

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Citations

1. Activation and Regulation of Blood V?2 T Cells Are Amplified by TREM-1during Active Pulmonary Tuberculosis
   Authors: Y Wu, YM Fang, L Ding, X Liu, NM Francisco, J Wen, C Liao, Z Ma, Z Li, M Li, S Ming, T Liu, M Zhang, M Wu, M Jacobs, S Gong, X Huang
   J. Immunol., 2018;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Urine TREM-1 as a marker of urinary tract infection in children
   Authors: E Sierra-Dia, A Bravo Cuél, PC Ortiz Laza, M García Gut, HF Georgina, R Anaya Prad
   J. Int. Med. Res., 2017;45(2):631-638.
   Species: Human
   Sample Type: Urine
   Application: Flow


3. TREM-1low is a novel characteristic for tumor-associated macrophages in lung cancer
   Oncotarget, 2016;7(26):40508-40517.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Differential pattern of cell-surface and soluble TREM-1 between sepsis and SIRS.
   Cytokine, 2013;61(1):112-7.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Role of MMPs in orchestrating inflammatory response in human monocytes via a TREM-1-PI3K-NF-kappaB pathway.
   J. Cell. Sci., 2012;91(6):933-45.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
   Authors: Bosco MC, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, Eva A, Novelli F, Cappello P, Giovarelli M, Varesio L
   Blood, 2011;117(9):2625-39.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Effects of TREM-1 activation in human neutrophils: activation of signaling pathways, recruitment into lipid rafts and association with TLR4.
   Authors: Fortin CF, Lesur O, Fulop T
   Int. Immunol., 2007;19(1):41-50.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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