抗Human CXCR5 Phycoerythrin抗体(Anti-Human CXCR5 Phycoerythrin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	NS0 mouse myeloma cell line transfected with human CXCR5Met1-Phe372Accession # P32302
Source	Monoclonal Mouse IgG2B Clone # 51505
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	10 µL/106 cells	See below

Flow Cytometry
	Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Mouse Anti-Human CXCR5 PE‑conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Flow Cytometry
	Detection of CXCR5 in CD4+ Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CXCR5 PE‑conjugated Monoclonal Antibody (Catalog # FAB190P) or (B) Mouse IgG2B isotype control antibody (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Related Product & Information

Entrez Gene IDs	643 (Human); 12145 (Mouse); 29363 (Rat)
Background	CXCR5
background_content	Background: CXCR5 CXCR5, also known as BLR-1, is a 7 transmembrane domain protein expressed on B cells. CXCR5 mediates B cell migration following binding of CXCL13.

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Citations

1. Follicular T Cells from smB(-) Common Variable Immunodeficiency Patients Are Skewed Toward a Th1 Phenotype
   Authors: V Cunill, A Clemente, N Lanio, C Barceló, V Andreu, J Pons, JM Ferrer
   Front Immunol, 2017;8(0):174.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
   Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
   PLoS ONE, 2015;10(3):e0120069.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Herpes simplex virus enhances chemokine function through modulation of receptor trafficking and oligomerization.
   Authors: Martinez-Martin N, Viejo-Borbolla A, Martin R, Blanco S, Benovic J, Thelen M, Alcami A
   Nat Commun, 2015;6(0):6163.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
   Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
   J Immunol, 2014;192(6):2634-42.
   


5. IL-12 directs further maturation of ex vivo differentiated NK cells with improved therapeutic potential.
   Authors: Lehmann, Dorit, Spanholtz, Jan, Sturtzel, Caterina, Tordoir, Marleen, Schlechta, Bernhard, Groenewegen, Dirk, Hofer, Erhard
   PLoS ONE, 2014;9(1):e87131.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
   Authors: Badr G, Lefevre EA, Mohany M
   PLoS ONE, 2011;6(9):e23741.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation.
   Authors: Barbosa RR, Silva SP, Silva SL, Melo AC, Pedro E, Barbosa MP, Pereira-Santos MC, Victorino RM, Sousa AE
   PLoS ONE, 2011;6(8):e22848.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
   Authors: Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
   Clin. Exp. Immunol., 2011;163(2):215-24.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
   Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
   J. Immunol., 2010;184(6):2886-98.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


10. Changes in chemokines and chemokine receptor expression on tonsillar B cells upon Epstein-Barr virus infection.
    Authors: Ehlin-Henriksson B, Liang W, Cagigi A, Mowafi F, Klein G, Nilsson A
    Immunology, 2009;127(4):549-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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