抗IgG 二次抗体(Anti-IgG, Goat, Donkey-Poly, HRP antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Goat
Label	HRP (Horseradish Peroxidase)
Immunogen	Goat IgG
Source	Polyclonal Donkey IgG
Purification	Antigen Affinity-purified
Specificity	Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	Use at a 1:1000 dilution.
Simple Western	Use at a 1:50 dilution.

Western Blot
	Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.
Simple Western
	Detection of Goat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Immunoglobulin G
Background	IgG
background_content	Background: IgG R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

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Citations

1. Re-evaluation of IL-10 signaling reveals novel insights on the contribution of the intracellular domain of the IL-10R2 chain
   Authors: RHP Wilbers, DR van Raaij, LB Westerhof, J Bakker, G Smant, A Schots
   PLoS ONE, 2017;12(10):e0186317.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Identification of the S100 fused-type protein hornerin as a regulator of tumor vascularity
   Authors: MF Gutknecht, ME Seaman, B Ning, DA Cornejo, E Mugler, PF Antkowiak, CA Moskaluk, S Hu, FH Epstein, KA Kelly
   Nat Commun, 2017;8(1):552.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Exosomes from embryonic mesenchymal stem cells alleviate osteoarthritis through balancing synthesis and degradation of cartilage extracellular matrix
   Authors: Y Wang, D Yu, Z Liu, F Zhou, J Dai, B Wu, J Zhou, BC Heng, XH Zou, H Ouyang, H Liu
   Stem Cell Res Ther, 2017;8(1):189.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Talin Modulation by a Synthetic N-Acylurea Derivative Reduces Angiogenesis in Human Endothelial Cells
   Authors: IR Lim, HJ Joo, M Jeong, JH Kim, SC Choi, C Kim, JW Jung, SJ Hong
   Int J Mol Sci, 2017;18(1):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Rebalancing gene haploinsufficiency in vivo by targeting chromatin
   Authors: Filomena Gabriella Fulcoli
   Nat Commun, 2016;7(0):11688.
   Species: Mouse
   Sample Type: Whole Cells
   Application: WB


6. Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation.
   Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M
   Sci Rep, 2015;5(0):9675.
   Species: Human
   Sample Type: Cell Lysates
   Application: Bioassay


7. Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort.
   Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill
   PLoS ONE, 2014;9(1):e85436.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole.
   Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao
   PLoS ONE, 2014;9(5):e97419.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


9. Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
   Authors: Shahrabi-Farahani, Shokoufe, Wang, Lili, Zwaans, Bernadet, Santana, Jeans M, Shimizu, Akio, Takashima, Seiji, Kreuter, Michael, Coultas, Leigh, D'Amore, Patricia, Arbeit, Jeffrey, Akslen, Lars A, Bielenberg, Diane R
   Lab Invest, 2014;94(7):752-65.
   Species: Mouse
   Sample Type: Whole Cells
   Application: WB


10. Cleavage of Histone 3 by Cathepsin D in the involuting mammary gland.
    Authors: Khalkhali-Ellis, Zhila, Goossens, William, Margaryan, Naira V, Hendrix, Mary J C
    PLoS ONE, 2014;9(7):e103230.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen

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