抗IL-15 Receptor α抗体(Anti-IL-15 Receptor α, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑15 R alpha Ile31-Thr172Accession # EAW86418
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-15 R alpha in Western blots. In this format, approximately 5% cross-reactivity with recombinant human IL‑2 sR gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑15 R alpha Fc Chimera (Catalog # 147-IR)

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Related Product & Information

Background

IL-15 R alpha

background_content

Background: IL-15 R alpha Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL‑15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL‑15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL‑15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL‑15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

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Citations

1. How a cytokine is chaperoned through the secretory pathway by complexing with its own receptor: lessons from interleukin-15 (IL-15)/IL-15 receptor alpha.
   Authors: Duitman EH, Orinska Z, Bulanova E, Paus R, Bulfone-Paus S
   Mol. Cell. Biol., 2008;28(15):4851-61.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


2. Tumor growth impedes natural-killer-cell maturation in the bone marrow.
   Authors: Richards JO, Chang X, Blaser BW, Caligiuri MA, Zheng P, Liu Y
   Blood, 2006;108(1):246-52.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


3. IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression.
   Authors: Koenen HJ, Fasse E, Joosten I
   J. Immunol., 2003;171(12):6431-41.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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