抗BMP-2/4抗体(Anti-BMP-2/4, Human, Goat-Poly, Biotin antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Biotin
Immunogen	E. coli-derived recombinant human BMP‑2Ala284-Arg396Accession # P12643
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human BMP‑2/BMP‑4 in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human BMP‑2 (Catalog # 355-BM) Recombinant Human BMP‑4 (Catalog # 314-BP)
Immunocytochemistry	5-15 µg/mL	Immersion fixed rat neonatal dorsal root ganglia cells

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Related Product & Information

Background

BMP-2/BMP-4

background_content

Background: BMP-2/BMP-4 Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).

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Citations

1. PLAP-1/asporin inhibits activation of BMP receptor via its leucine-rich repeat motif.
   Authors: Tomoeda M, Yamada S, Shirai H, Ozawa Y, Yanagita M, Murakami S
   Biochem. Biophys. Res. Commun., 2008;371(2):191-6.
   Species: Human
   Sample Type: Complex Sample Type
   Application: WB


2. Baalc, a marker of mesoderm and muscle.
   Authors: Satoskar AA, Tanner SM, Weinstein M, Qualman SJ, de la Chapelle A
   Gene Expr. Patterns, 2005;5(4):463-73.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC

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