抗SOX17抗体(Anti-SOX17, Human, Goat-Poly antibody)

掲載日情報:2018/11/26 現在Webページ番号:27385

SOX17に対する抗体(Anti-SOX17, Human, Goat-Poly )です。
本製品は研究用です。研究用以外には使用できません。

価格

[在庫・価格 :2024年04月23日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
Anti-SOX17, Human, Goat-Poly
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 212
説明文
別名:FLJ22252
Genbank No: 64321
Protein Accession No: Q9H6I2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ChIP,IC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事 StemXVivo Endoderm / Ectoderm / Mesoderm Kit
Anti-SOX17 Antibody
関連記事
Anti-Human SOX17 Affinity Purified Polyclonal Ab
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 85
説明文
Simple Western対応抗体。※受注発注品。形状:溶液または凍結乾燥
別名:FLJ22252
Genbank No: 64321
Protein Accession No: Q9H6I2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat クラス IgG 標識 Unlabeled
交差性 Human 適用 ChIP,IC,Simple Western,Western Blot
クロナリティ Polyclonal フォーマット 性状 Antigen Affinity Purified 吸収処理
掲載カタログ

製品記事
関連記事

[在庫・価格 :2024年04月23日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-SOX17, Human, Goat-Poly

文献数: 212

説明文 別名:FLJ22252
Genbank No: 64321
Protein Accession No: Q9H6I2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 Human 免疫動物 Goat
交差性 Human 適用 ChIP,IC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事 StemXVivo Endoderm / Ectoderm / Mesoderm Kit
Anti-SOX17 Antibody
関連記事

Anti-Human SOX17 Affinity Purified Polyclonal Ab

文献数: 85

説明文 Simple Western対応抗体。※受注発注品。形状:溶液または凍結乾燥
別名:FLJ22252
Genbank No: 64321
Protein Accession No: Q9H6I2
別包装品 別包装品あり
法規制等
保存条件 -20℃ 法規備考
抗原種 免疫動物 Goat
交差性 Human 適用 ChIP,IC,Simple Western,Western Blot
標識 Unlabeled 性状 Antigen Affinity Purified
吸収処理 クラス IgG
クロナリティ Polyclonal フォーマット
掲載カタログ

製品記事
関連記事



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Product Details

Species ReactivityHuman
LabelUnconjugated
ImmunogenE. coli-derived recombinant human SOX17Asp177-Val414Accession # Q9H6I2
SourcePolyclonal Goat IgG
PurificationAntigen Affinity-purified
SpecificityDetects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLSee below
Simple Western10 µg/mLSee below
Chromatin Immunoprecipitation (ChIP)5 µg/5 x 106 cellsSee below
Immunocytochemistry5-15 µg/mLSee below


Western Blot
Detection of Human SOX17 by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation.
Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Immunocytochemistry
SOX17 in B16 Mouse Cell Line.
SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
SOX17 in Human BG01V Cells.
SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
Detection of Human SOX17 by Simple WesternTM.
Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western
Detection of Human SOX17 by Simple WesternTM.
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.


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Related Product & Information

BackgroundSOX17
background_contentBackground:
SOX17
SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
    Authors: JJ Zylicz, M Borensztei, FC Wong, Y Huang, C Lee, S Dietmann, MA Surani
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC

  2. Establishment of STUB1/CHIP mutant induced pluripotent stem cells (iPSCs) from a patient with Gordon Holmes syndrome/SCAR16
    Authors: S Schuster, Y Schelling, M Synofzik, P Höflinger, L Schöls, S Hauser
    Stem Cell Res, 2018;29(0):166-169.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  3. Induced pluripotent stem cells derived from a schizophrenia patient with ASTN2 deletion
    Authors: Y Arioka, I Kushima, H Kubo, D Mori, N Ozaki
    Stem Cell Res, 2018;30(0):81-84.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC

  4. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
    Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
    Cell Stem Cell, 2018;22(4):543-558.e12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

  5. Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free conditions
    Authors: A Sevilla, E Forero, M Zimmer, H Martinez, K Reggio, D Paull, D Egli, S Noggle
    Stem Cell Res, 2018;29(0):99-102.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC

  6. Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells
    Authors: MT De Angelis, EI Parrotta, G Santamaria, G Cuda
    Cell Death Dis, 2018;9(1):6.
    Species: Human
    Sample Type: Embryoid Bodies
    Application: ICC

  7. Generation of two induced pluripotent stem cell (iPSC) lines from p.F508del Cystic Fibrosis patients
    Authors: A Fleischer, IM Lorenzo, E Palomino, T Aasen, F Gómez, M Servera, VJ Asensio, V Gálvez, JC Izpisúa-Be, D Bachiller
    Stem Cell Res, 2018;29(0):1-5.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  8. Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture
    Authors: A Arauchi, K Matsuura, T Shimizu, T Okano
    Front Endocrinol (Lausanne), 2017;8(0):103.
    Species: Human
    Sample Type: Embryo Bodies
    Application: Flow

  9. Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes
    Authors: Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura
    J Diabetes Investig, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

  10. Guided self-organization and cortical plate formation in human brain organoids
    Authors: MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich
    Nat. Biotechnol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC



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