抗SOX17抗体(Anti-SOX17, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human SOX17Asp177-Val414Accession # Q9H6I2
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Chromatin Immunoprecipitation (ChIP)	5 µg/5 x 106 cells	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human SOX17 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
	Detection of SOX17-regulated Genes by Chromatin Immunoprecipitation. Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The p21 promoter was detected by standard PCR.
Immunocytochemistry
	SOX17 in B16 Mouse Cell Line. SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	SOX17 in Human BG01V Cells. SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Human SOX17 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Simple Western
	Detection of Human SOX17 by Simple WesternTM. Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background	SOX17
background_content	Background: SOX17 SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

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Citations

1. G9a regulates temporal preimplantation developmental program and lineage segregation in blastocyst
   Authors: JJ Zylicz, M Borensztei, FC Wong, Y Huang, C Lee, S Dietmann, MA Surani
   Elife, 2018;7(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Establishment of STUB1/CHIP mutant induced pluripotent stem cells (iPSCs) from a patient with Gordon Holmes syndrome/SCAR16
   Authors: S Schuster, Y Schelling, M Synofzik, P Höflinger, L Schöls, S Hauser
   Stem Cell Res, 2018;29(0):166-169.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Induced pluripotent stem cells derived from a schizophrenia patient with ASTN2 deletion
   Authors: Y Arioka, I Kushima, H Kubo, D Mori, N Ozaki
   Stem Cell Res, 2018;30(0):81-84.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC


4. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation
   Authors: NS Corsini, AM Peer, P Moeseneder, M Roiuk, TR Burkard, HC Theussl, I Moll, JA Knoblich
   Cell Stem Cell, 2018;22(4):543-558.e12.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. Derivation and characterization of the NIH registry human stem cell line NYSCF100 line under defined feeder-free conditions
   Authors: A Sevilla, E Forero, M Zimmer, H Martinez, K Reggio, D Paull, D Egli, S Noggle
   Stem Cell Res, 2018;29(0):99-102.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC


6. Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells
   Authors: MT De Angelis, EI Parrotta, G Santamaria, G Cuda
   Cell Death Dis, 2018;9(1):6.
   Species: Human
   Sample Type: Embryoid Bodies
   Application: ICC


7. Generation of two induced pluripotent stem cell (iPSC) lines from p.F508del Cystic Fibrosis patients
   Authors: A Fleischer, IM Lorenzo, E Palomino, T Aasen, F Gómez, M Servera, VJ Asensio, V Gálvez, JC Izpisúa-Be, D Bachiller
   Stem Cell Res, 2018;29(0):1-5.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Functional Thyroid Follicular Cells Differentiation from Human-Induced Pluripotent Stem Cells in Suspension Culture
   Authors: A Arauchi, K Matsuura, T Shimizu, T Okano
   Front Endocrinol (Lausanne), 2017;8(0):103.
   Species: Human
   Sample Type: Embryo Bodies
   Application: Flow


9. Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes
   Authors: Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura
   J Diabetes Investig, 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. Guided self-organization and cortical plate formation in human brain organoids
    Authors: MA Lancaster, NS Corsini, S Wolfinger, EH Gustafson, AW Phillips, TR Burkard, T Otani, FJ Livesey, JA Knoblich
    Nat. Biotechnol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC

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