抗MIP-3β抗体(Anti-MIP-3β, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL19/MIP-3 beta Gly26-Val107 (Ser108LeuGlu)Accession # Q548P0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant rat CCL19/MIP-3 beta is observed and less than 10% cross-reactivity with recombinant human CCL19/MIP‑3 beta is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL19/MIP‑3 beta (Catalog # 440-M3)
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis inthe BaF3 mouse pro-B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 0.8-4 μg/mL in the presence of 50 ng/mL Recombinant Mouse CCL19/MIP‑3 beta.

Neutralization
	Chemotaxis Induced by CCL19/MIP‑3 beta and Neutralization by Mouse CCL19/MIP‑3 beta Antibody. Recombinant Mouse CCL19/MIP‑3 beta (Catalog #440-M3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line).The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL19/MIP‑3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880). The ND50 is typically 0.8-4 μg/mL.
Immunocytochemistry
	CCL19/MIP‑3 beta in Mouse Splenocytes. CCL19/MIP‑3 beta was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
	CCL19/MIP‑3 beta in Mouse Dendritic Cells. CCL19/MIP‑3 beta was detected in immersion fixed mouse dendritic cells using Goat Anti-Mouse CCL19/MIP‑3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Entrez Gene IDs	6363 (Human); 24047 (Mouse); 362506 (Rat)
Background	CCL19/MIP-3 beta
background_content	Background: CCL19/MIP-3 beta CCL19/MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is a reported beta  chemokine that binds specifically to the chemokine receptor CCR-7/EBI-1/BLR-2. Mouse (human) MIP-3 beta cDNA encodes a 108 (98) amino acid residue precursor protein with a predicted 25 (21) aa residue signal peptide that is cleaved to form the 83 (77) aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity). Mouse MIP-3 beta shares 83% aa sequence homology with human MIP-3 beta. MIP-3 beta  has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix, and spleen) in dendritic cells within the T cell zone. The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for T cells and B cells. The MIP-3 beta receptor (CCR7/EBI-1/BLR-2) is expressed in various lymphoid tissues and activated B and T lymphocytes. CCR7 is also strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpes virus 6 or 7.

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Citations

1. CCR7-CCL19/CCL21 Axis is Essential for Effective Arteriogenesis in a Murine Model of Hindlimb Ischemia
   Authors: AY Nossent, AJ Bastiaanse, EA Peters, MR de Vries, Z Aref, SM Welten, SC de Jager, TC van der Po, PH Quax
   J Am Heart Assoc, 2017;6(3):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


2. Deficiency of lymph node-resident dendritic cells (DCs) and dysregulation of DC chemoattractants in a malnourished mouse model of Leishmania donovani infection.
   Authors: Ibrahim M, Barnes J, Osorio E, Anstead G, Jimenez F, Osterholzer J, Travi B, Ahuja S, White A, Melby P
   Infect Immun, 2014;82(8):3098-112.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
   Authors: Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS
   J. Immunol., 2010;184(10):5571-81.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice.
   Authors: Demoor T, Bracke KR, Vermaelen KY, Dupont L, Joos GF, Brusselle GG
   J. Immunol., 2009;183(12):8186-94.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


6. CCR7-CCL19/CCL21-regulated dendritic cells are responsible for effectiveness of sublingual vaccination.
   Authors: Song JH, Kim JI, Kwon HJ, Shim DH, Parajuli N, Cuburu N, Czerkinsky C, Kweon MN
   J. Immunol., 2009;182(11):6851-60.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. CCR7 ligands stimulate the intranodal motility of T lymphocytes in vivo.
   Authors: Worbs T, Mempel TR, Bolter J, von Andrian UH, Forster R
   J. Exp. Med., 2007;204(3):489-95.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
   Authors: Ato M, Stager S, Engwerda CR, Kaye PM
   Nat. Immunol., 2002;3(12):1185-91.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Fresh/Frozen

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