抗p38抗体(Anti-p38 MAPK, Phosphorylated, Human/Mouse/Rat, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Phosphopeptide containing human, rat and mouse p38 alpha T180/Y182 site
Source	Polyclonal Rabbit IgG
Purification	Antigen and protein A Affinity-purified
Specificity	Detects human, mouse and rat Phospho-p38 alpha  MAP Kinase when dually phosphorylated at T180/Y182. May also recognize the related phosphorylated isoforms p38 beta, p38 gamma and p38δ.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human Phospho-p38 MAP Kinase (T180/Y182) by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IL‑1 beta (Catalog # 201-LB) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.). p38 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Human Phospho-p38 MAP Kinase (T180/Y182) by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with ultraviolet light (UV), loaded at 0.5 mg/mL. A specific band was detected for Phospho-p38 MAP Kinase (T180/Y182) at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

p38

background_content

Background: p38 The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

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Citations

1. ADAM8 as a drug target in pancreatic cancer.
   Authors: Schlomann U, Koller G, Conrad C, Ferdous T, Golfi P, Garcia A, Hofling S, Parsons M, Costa P, Soper R, Bossard M, Hagemann T, Roshani R, Sewald N, Ketchem R, Moss M, Rasmussen F, Miller M, Lauffenburger D, Tuveson D, Nimsky C, Bartsch J
   Nat Commun, 2015;6(0):6175.
   Species: Human
   Sample Type: Whole Cells
   Application: Blocking


2. Molecular mechanisms underlying synergistic adhesion of sickle red blood cells by hypoxia and low nitric oxide bioavailability.
   Authors: Gutsaeva, Diana R, Montero-Huerta, Pedro, Parkerson, James B, Yerigenahally, Shobha D, Ikuta, Tohru, Head, C Alvin
   Blood, 2014;123(12):1917-26.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. Pre-exposure of Mycobacterium tuberculosis-infected macrophages to crystalline silica impairs control of bacterial growth by deregulating the balance between apoptosis and necrosis.
   Authors: Chavez-Galan L, Ramon-Luing L, Torre-Bouscoulet L, Perez-Padilla R, Sada-Ovalle I
   PLoS ONE, 2013;8(11):e80971.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Inhibition of Doxorubicin-Induced Senescence by PPARdelta Activation Agonists in Cardiac Muscle Cells: Cooperation between PPARdelta and Bcl6.
   Authors: Altieri P, Spallarossa P, Barisione C, Garibaldi S, Garuti A, Fabbi P, Ghigliotti G, Brunelli C
   PLoS ONE, 2012;7(9):e46126.
   Species: Rat
   Sample Type: Cell Lysates
   Application: WB


5. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
   BMC Neurosci, 2012;13(0):50.
   Species: Rat
   Sample Type: Tissue Homogenates
   Application: WB


6. Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane.
   Authors: Tang WH, Stitham J, Gleim S, Di Febbo C, Porreca E, Fava C, Tacconelli S, Capone M, Evangelista V, Levantesi G, Wen L, Martin K, Minuz P, Rade J, Patrignani P, Hwa J
   J. Clin. Invest., 2011;121(11):4462-76.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer.
   Authors: Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM
   Nature, 2010;468(7320):98-102.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


8. The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages.
   Authors: Lane AE, Tan JT, Hawkins CL
   Biochem. J., 2010;430(1):161-9.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Colocalization of the VEGF-R2 and the common IL-3/GM-CSF receptor beta chain to lipid rafts leads to enhanced p38 activation.
   Authors: Saulle E, Riccioni R, Coppola S, Parolini I, Diverio D, Riti V, Mariani G, Laufer S, Sargiacomo M, Testa U
   Br. J. Haematol., 2009;145(3):399-411.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. MAP-ing glioma invasion: mitogen-activated protein kinase kinase 3 and p38 drive glioma invasion and progression and predict patient survival.
    Authors: Demuth T, Reavie LB, Rennert JL, Nakada M, Nakada S, Hoelzinger DB, Beaudry CE, Henrichs AN, Anderson EM, Berens ME
    Mol. Cancer Ther., 2007;6(4):1212-22.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC

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