抗PTEN抗体(Anti-PTEN, Human, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Human PTEN synthetic peptideSer385-Val403Accession # P60484
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Detects human, mouse, and rat PTEN in Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Simple Western	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	See below

Western Blot
	Detection of Human/Mouse/Rat PTEN by Western Blot. Western blot shows lysates of mouse and rat brain tissue, A431 human epithelial carcinoma cell line, and MRC-5 human embryonic lung fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human PTEN (5 ng) was included. A specific band for PTEN was detected at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Immunohistochemistry
	PTEN in Human Liver. PTEN was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Intracellular Staining by Flow Cytometry
	Detection of PTEN in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF847, filled histogram) or control antibody (Catalog # AB‑105‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Simple Western
	Detection of Human PTEN by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PTEN at approximately 60 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat PTEN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF847). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

PTEN

background_content

Background: PTEN The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).

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Citations

1. Biomarker analysis of the NeoSphere study: pertuzumab, trastuzumab, and docetaxel versus trastuzumab plus docetaxel, pertuzumab plus trastuzumab, or pertuzumab plus docetaxel for the neoadjuvant treatment of HER2-positive breast cancer
   Authors: G Bianchini, A Kiermaier, GV Bianchi, YH Im, T Pienkowski, MC Liu, LM Tseng, M Dowsett, L Zabaglo, S Kirk, T Szado, J Eng-Wong, LC Amler, P Valagussa, L Gianni
   Breast Cancer Res, 2017;19(1):16.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. TGF-? induces phosphorylation of phosphatase and tensin homolog: implications for fibrosis of the trabecular meshwork tissue in glaucoma
   Authors: N Tellios, JC Belrose, AC Tokarewicz, C Hutnik, H Liu, A Leask, M Motolko, M Iijima, SK Parapuram
   Sci Rep, 2017;7(1):812.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. A novel role for GSK3? as a modulator of Drosha microprocessor activity and MicroRNA biogenesis
   Nucleic Acids Res, 2016;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Tumor suppressor PTEN in breast cancer: heterozygosity, mutations and protein expression.
   Authors: Kechagioglou, Petros, Papi, Rigini M, Provatopoulou, Xeni, Kalogera, Eleni, Papadimitriou, Elli, Grigoropoulos, Petros, Nonni, Aphrodit, Zografos, George, Kyriakidis, Dimitrio, Gounaris, Antonia
   Anticancer Res, 2014;34(3):1387-400.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Basal activation of p70S6K results in adipose-specific insulin resistance in protein-tyrosine phosphatase 1B -/- mice.
   Authors: Ruffolo SC, Forsell PK, Yuan X, Desmarais S, Himms-Hagen J, Cromlish W, Wong KK, Kennedy BP
   J. Biol. Chem., 2007;282(42):30423-33.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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