抗Caspase-3抗体(Anti-Caspase-3, Active, Human/Mouse, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	KLH-coupled human Caspase-3 synthetic peptideCRGTELDCGIETDAccession # P42574
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Distinguishes apoptotic cells from non-apoptotic cells in immunohistochemistry. Detects the p17 subunit of Caspase-3, but does not detect the precursor form in Western blot. We recommend R&D Systems Affinity Purified Anti-Caspase-3 (Catalog # AF-605-NA) or Monoclonal Anti-Cleaved-Caspase-3 (Catalog # MAB835) for Western blot applications.

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Applications and Data

	Recommended Concentration	Sample
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	Caspase‑3 in Jurkat Human Cell Line. Caspase‑3 was detected in immersion fixed anti-FAS treated Jurkat human acute T cell leukemia cell line using 0.3 µg/mL Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Caspase‑3 in Jurkat Human Cell Line. Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line stimulated with staurosporin using Human/Mouse Active Caspase‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (yellow; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Related Product & Information

Entrez Gene IDs	836 (Human); 12367 (Mouse)
Background	Caspase-3
background_content	Background: Caspase-3 Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7). Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

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Citations

1. PRC1 Fine-tunes Gene Repression and Activation to Safeguard Skin Development and Stem Cell Specification
   Authors: I Cohen, D Zhao, C Bar, VJ Valdes, KL Dauber-Dec, MB Nguyen, M Nakayama, M Rendl, WA Bickmore, H Koseki, D Zheng, E Ezhkova
   Cell Stem Cell, 2018;22(5):726-739.e7.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. A motif within the armadillo repeat of Parkinson's-linked LRRK2 interacts with FADD to hijack the extrinsic death pathway
   Authors: N Antoniou, D Vlachakis, A Memou, E Leandrou, PE Valkimadi, K Melachroin, DB Re, S Przedborsk, WT Dauer, L Stefanis, HJ Rideout
   Sci Rep, 2018;8(1):3455.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Synapsin I and Synapsin II regulate neurogenesis in the dentate gyrus of adult mice
   Authors: R Barbieri, A Contestabi, MG Ciardo, N Forte, A Marte, P Baldelli, F Benfenati, F Onofri
   Oncotarget, 2018;9(27):18760-18774.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-F


4. Tracking cellular and molecular changes in a species-specific manner during experimental tumor progression in vivo
   Authors: E Indersie, KB Hooks, C Capdeviell, M Fabre, N Dugot-Sena, A Desplat, S Lepreux, A Merched, CF Grosset, M Hagedorn
   Oncotarget, 2018;9(22):16149-16162.
   Species: Human
   Sample Type: Complex Sample Type
   Application: IHC


5. The kallikrein-Kinin system modulates the progression of colorectal liver metastases in a mouse model
   Authors: PLN da Costa, D Wynne, T Fifis, L Nguyen, M Perini, C Christophi
   BMC Cancer, 2018;18(1):382.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Intermittent, low dose carbon monoxide exposure enhances survival and dopaminergic differentiation of human neural stem cells
   Authors: N Dreyer-And, AS Almeida, P Jensen, M Kamand, J Okarmus, T Rosenberg, SD Friis, A Martínez S, M Blaabjerg, BW Kristensen, T Skrydstrup, JB Gramsberge, HLA Vieira, M Meyer
   PLoS ONE, 2018;13(1):e0191207.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Therapeutic effects of telomerase in mice with pulmonary fibrosis induced by damage to the lungs and short telomeres
   Authors: JM Povedano, P Martinez, R Serrano, Á Tejera, G Gómez-Lópe, M Bobadilla, JM Flores, F Bosch, MA Blasco
   Elife, 2018;7(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


8. Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium
   Authors: LA Murray, R Dunmore, A Camelo, CA Da Silva, MJ Gustavsson, DM Habiel, TL Hackett, CM Hogaboam, MA Sleeman, DA Knight
   Respir. Res., 2017;18(1):82.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


9. Glycogen synthase kinase-3 inhibition sensitizes human induced pluripotent stem cells to thiol-containing antioxidants induced apoptosis
   Authors: C Tu, R Xu, M Koleti, J Zoldan
   Stem Cell Res, 2017;23(0):182-187.
   Species: Human
   Sample Type: Whole Cells
   Application: Functional Assay


10. New tumor suppressor microRNAs target glypican-3 in human liver cancer
    Authors: F Cartier, E Indersie, S Lesjean, J Charpentie, KB Hooks, A Ghousein, A Desplat, N Dugot-Sena, V Trézéguet, F Sagliocco, M Hagedorn, CF Grosset
    Oncotarget, 2017;0(0):.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: IHC

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