抗VCAM-1抗体(Anti-VCAM-1, Human, Sheep-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line (CHO)-derived recombinant human VCAM-1Extracellular domain
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects VCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse VCAM-1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Adhesion Blockade	The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human VCAM-1/CD106 (Catalog # ADP5, 2.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.

Western Blot

Detection of Human VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VCAM‑1/CD106 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Vascular Cell Adhesion Molecule 1
Entrez Gene IDs	7412 (Human); 22329 (Mouse)
Background	VCAM-1/CD106
background_content	Background: VCAM-1/CD106 VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha, and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

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Citations

1. N-octanoyl-dopamine is an agonist at the capsaicin receptor TRPV1 and mitigates is chemia-induced acute kidney injury in rat.
   Authors: Tsagogiorgas C, Wedel J, Hottenrott M, Schneider M, Binzen U, Greffrath W, Treede R, Theisinger B, Theisinger S, Waldherr R, Kramer B, Thiel M, Schnuelle P, Yard B, Hoeger S
   PLoS ONE, 2012;7(8):e43525.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Mesenchymal Stem Cells Transmigrate Between and Directly Through Tumor Necrosis Factor-alpha-Activated Endothelial Cells Via Both Leukocyte-Like and Novel Mechanisms.
   Authors: Teo G, Ankrum J, Martinelli R, Boetto S, Simms K, Sciuto T, Dvorak A, Karp J, Carman C
   Stem Cells, 2012;30(11):2472-86.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


3. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
   Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
   J. Immunol. Methods, 2012;378(1):72-80.
   Species: Human
   Sample Type: Serum
   Application: ELISA Development


4. The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
   Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
   Arthritis Rheum., 2007;56(6):1817-26.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
   Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
   Blood, 2006;107(6):2252-61.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
   Authors: Carman CV, Springer TA
   J. Cell Biol., 2004;167(2):377-88.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
   Authors: Carman CV, Springer TA
   J. Cell Biol., 2004;167(2):377-88.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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