抗PECAM-1抗体(Anti-PECAM-1, Human, Sheep-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human CD31Extracellular domain
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Human CD31/PECAM‑1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	CD31/PECAM‑1 in HUVECs. CD31/PECAM‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western
	Detection of Human CD31/PECAM‑1 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD31/PECAM‑1 at approximately173-186 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Long Name	Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs	5175 (Human); 18613 (Mouse); 29583 (Rat)
Background	CD31/PECAM-1
background_content	Background: CD31/PECAM-1 The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

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Citations

1. Leucine-Rich ?-2-Glycoprotein-1 (LRG-1) Expression in Retinoblastoma
   Authors: R Amer, L Tiosano, J Pe'er
   Invest. Ophthalmol. Vis. Sci., 2018;59(2):685-692.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


2. Tissue-engineered 3D human lymphatic microvascular network for in vitro studies of lymphangiogenesis
   Authors: L Gibot, T Galbraith, J Bourland, A Rogic, M Skobe, FA Auger
   Nat Protoc, 2017;12(5):1077-1088.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells
   Sci Rep, 2016;6(0):35680.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fluorescence


4. Aberrant bispecific antibody pharmacokinetics linked to liver sinusoidal endothelium clearance mechanism in cynomolgus monkeys
   Authors: A Datta-Mann, JE Croy, L Schirtzing, S Torgerson, M Breyer, VJ Wroblewski
   MAbs, 2016;8(5):969-82.
   


5. TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis.
   Authors: Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J
   Oncogene, 2015;35(6):748-60.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Not Specified


6. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
   Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
   Reprod Biol Endocrinol, 2015;12(0):123.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology.
   Authors: Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L
   PLoS ONE, 2015;10(4):e0125697.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Fresh


8. Consumption of a Western-style diet during pregnancy impairs offspring islet vascularization in a Japanese macaque model.
   Authors: Pound L, Comstock S, Grove K
   Am J Physiol Endocrinol Metab, 2014;307(1):E115-23.
   Species: Primate - Macaca mulatta (Rhesus Macaque)
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA.
   Authors: Blosser, Wayne, Vakana, Eliza, Wyss, Lisa V, Swearingen, Michelle, Stewart, Julie, Stancato, Louis, Tate, Courtney
   PLoS ONE, 2014;9(4):e96036.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


10. miR-27a regulates endothelial differentiation of breast cancer stem like cells.
    Authors: Tang, W, Yu, F, Yao, H, Cui, X, Jiao, Y, Lin, L, Chen, J, Yin, D, Song, E, Liu, Q
    Oncogene, 2014;33(20):2629-38.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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