抗Tie-2抗体(Anti-Tie-2, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse, Rat
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Tie‑2Ala23-Lys744Accession # CAA47857
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse and rat Tie-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) Tie‑2 is observed and less than 1% cross-reactivity with recombinant zebrafish Tie-2 and rhTie-1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse Tie‑2 Fc Chimera (Catalog # 762-T2) and Recombinant Rat Tie-2 Fc Chimera (Catalog # 3874-T2)
Blockade of Receptor-ligand Interaction	In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of biotinylated Recombinant Human Angiopoietin-2(Catalog # BT623) to immobilized Recombinant Mouse Tie-2 Fc Chimera (Catalog # 762-T2) coated at 4 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.

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Related Product & Information

Long Name	Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Entrez Gene IDs	7010 (Human); 21687 (Mouse); 30747 (Zebrafish)
Background	Tie-2
background_content	Background: Tie-2 Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

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Citations

1. Tie2 protects the vasculature against thrombus formation in systemic inflammation
   Authors: SJ Higgins, K Ceunynck, J Kellum, X Chen, X Gu, SA Chaudhry, S Schulman, TA Libermann, S Lu, NI Shapiro, DC Christiani, R Flaumenhaf, SM Parikh
   J. Clin. Invest., 2018;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
   Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
   J. Clin. Invest., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling
   Authors: N Morooka, S Futaki, R Sato-Nishi, M Nishino, Y Totani, C Shimono, I Nakano, H Nakajima, N Mochizuki, K Sekiguchi
   Circ. Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. Endothelial destabilization by angiopoietin-2 via integrin beta1 activation.
   Authors: Hakanpaa L, Sipila T, Leppanen V, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
   Nat Commun, 2015;6(0):5962.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IF


5. Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina.
   Authors: Zhou Y, Williams J, Smallwood P, Nathans J
   PLoS ONE, 2015;10(12):e0143650.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Frozen


6. COMP-angiopoietin-1 recovers molecular biomarkers of neuropathy and improves vascularisation in sciatic nerve of ob/ob mice.
   Authors: Kosacka J, Nowicki M, Kloting N, Kern M, Stumvoll M, Bechmann I, Serke H, Bluher M
   PLoS ONE, 2012;7(3):e32881.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


7. Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway.
   Authors: Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J
   Biochem. Biophys. Res. Commun., 2009;378(2):296-301.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


8. Immunotherapy of tumors with protein vaccine based on chicken homologous Tie-2.
   Authors: Luo Y, Wen YJ, Ding ZY, Fu CH, Wu Y, Liu JY, Li Q, He QM, Zhao X, Jiang Y, Li J, Deng HX, Kang B, Mao YQ, Wei YQ
   Clin. Cancer Res., 2006;12(6):1813-9.
   Species: Chicken
   Sample Type: Recombinant Protein
   Application: WB


9. A neurovascular niche for neurogenesis after stroke.
   Authors: Ohab JJ, Fleming S, Blesch A, Carmichael ST
   J. Neurosci., 2006;26(50):13007-16.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo

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