抗Flt-4抗体(Anti-Flt-4, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF R3/Flt-4Tyr25-Asp770Accession # P35917
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse VEGF R3/Flt-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF R3, 10% cross-reactivity with rhVEGF R2, and 5% cross-reactivity with rhVEGF R1.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse VEGF R3/Flt‑4 Fc Chimera (Catalog # 743-R3)
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry
	Detection of VEGF R3/Flt‑4 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGF R3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

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Related Product & Information

Background

VEGF R3/Flt-4

background_content

Background: VEGF R3/Flt-4 VEGF R3 (Flt-4), together with VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. In adults, VEGF R3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGF R3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGF R3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGF R3 has been reported to serve as the receptors for VEGF-C and VEGF-D.

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Citations

1. Expression of the Atypical Chemokine Receptor ACKR4 Identifies a Novel Population of Intestinal Submucosal Fibroblasts That Preferentially Expresses Endothelial Cell Regulators
   Authors: CA Thomson, SA van de Pav, M Stakenborg, E Labeeuw, G Matteoli, AM Mowat, RJB Nibbs
   J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Heterogeneity in VEGFR3 levels drives lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms
   Authors: Y Zhang, MH Ulvmar, L Stanczuk, I Martinez-C, M Frye, K Alitalo, T Mäkinen
   Nat Commun, 2018;9(1):1296.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


3. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
   Authors: M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen
   Nat Commun, 2018;9(1):1511.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling
   Authors: K Heinolaine, S Karaman, G D'Amico, T Tammela, R Sormunen, L Eklund, K Alitalo, G Zarkada
   Circ. Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


5. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma
   Authors: J Kim, DY Park, H Bae, DY Park, D Kim, CK Lee, S Song, TY Chung, DH Lim, Y Kubota, YK Hong, Y He, HG Augustin, G Oliver, GY Koh
   J. Clin. Invest., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
   Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
   Sci Rep, 2017;7(1):4916.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Whole-body imaging of lymphovascular niches identifies pre-metastatic roles of midkine
   Authors: D Olmeda, D Cerezo-Wal, E Riveiro-Fa, PC Pennacchi, M Contreras-, N Ibarz, M Cifdaloz, X Catena, TG Calvo, E Cañón, D Alonso-Cur, J Suarez, L Osterloh, O Graña, F Mulero, D Megías, M Cañamero, JL Martínez-T, C Mondal, J Di Martino, D Lora, I Martinez-C, JJ Bravo-Cord, J Muñoz, S Puig, P Ortiz-Rome, JL Rodriguez-, S Ortega, MS Soengas
   Nature, 2017;546(7660):676-680.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC paraffin embedded


8. Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction
   Authors: F Tatin, E Renaud-Gab, AC Godet, F Hantelys, F Pujol, F Morfoisse, D Calise, F Viars, P Valet, B Masri, AC Prats, B Garmy-Susi
   JCI Insight, 2017;2(12):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. FGF-dependent metabolic control of vascular development
   Authors: P Yu, K Wilhelm, A Dubrac, JK Tung, TC Alves, JS Fang, Y Xie, J Zhu, Z Chen, F De Smet, J Zhang, SW Jin, L Sun, H Sun, RG Kibbey, KK Hirschi, N Hay, P Carmeliet, TW Chittenden, A Eichmann, M Potente, M Simons
   Nature, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Vitamin D inhibits lymphangiogenesis through VDR-dependent mechanisms
    Authors: S Yazdani, F Poosti, L Toro, J Wedel, R Mencke, K Mirkovi?, MH de Borst, JS Alexander, G Navis, H van Goor, J van den Bo, JL Hillebrand
    Sci Rep, 2017;7(0):44403.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC

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