抗ICAM-1抗体(Anti-ICAM-1, Human, Sheep-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human ICAM-1Extracellular domain
Source	Polyclonal Sheep IgG
Purification	Antigen Affinity-purified
Specificity	Selected for its ability to block the adhesion of HSB‑2 cells to immobilized recombinant human (rh) ICAM‑1. In direct ELISAs, approximately 5% cross-reactivity with rhICAM-3 is observed and less than 2% cross-reactivity with rhICAM-2, rhICAM-4, rhICAM-5, recombinant rat ICAM‑1, recombinant mouse ICAM‑1, and rhVCAM-1 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Adhesion Blockade	The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized recombinant human ICAM-1/Fc Chimera (12.5 μg/mL, 100 µL/well) was maximally inhibited (80‑100%) by 25 μg/mL of the antibody.

Western Blot

Detection of Human ICAM‑1/CD54 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA) for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF720) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Long Name	Intercellular Adhesion Molecule 1
Entrez Gene IDs	3383 (Human); 15894 (Mouse); 25464 (Rat)
Background	ICAM-1/CD54
background_content	Background: ICAM-1/CD54 Intercellular Adhesion Molecule-1 (ICAM-1) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, LAD, cancer and transplantation.

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Citations

1. Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction
   Genome Biol, 2016;17(1):188.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC Fluorescence


2. High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
   Authors: Hashizume M, Mihara M
   Biochem. Biophys. Res. Commun., 2010;403(2):184-9.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
   Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
   J. Leukoc. Biol., 2008;84(4):1109-19.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
   Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E
   Lab. Invest., 2007;87(11):1159-70.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Shear flow-dependent integration of apical and subendothelial chemokines in T-cell transmigration: implications for locomotion and the multistep paradigm.
   Authors: Schreiber TH, Shinder V, Cain DW, Alon R, Sackstein R
   Blood, 2007;109(4):1381-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
   Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
   Arthritis Rheum., 2007;56(6):1817-26.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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