抗ATM抗体(Anti-ATM, Phosphorylated, Rabbit-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse, Rat
Label	Unconjugated
Immunogen	Phosphopeptide containing human ATM S1981 site
Source	Polyclonal Rabbit IgG
Purification	Antigen Affinity-purified
Specificity	Detects human ATM when phosphorylated at S1981. Also detects the comparable phosphorylated sites in mouse ATM (S1987) and rat ATM (S1952).

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below

Western Blot
	Detection of Human/Mouse/Rat Phospho-ATM (S1981) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-ATM (S1981) was detected at approximately 370 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda ‑phosphatase ( lambda -PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human Phospho-ATM (S1981) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM Camptothecin (CPT) for 1 hour, loaded at 0.2 mg/mL. A specific band was detected for Phospho-ATM (S1981) at approximately 270 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-ATM (S1981) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1655). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

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Related Product & Information

Long Name	Ataxia Telangiectasia-mutated
Background	ATM
background_content	Background: ATM The Ataxia telangiectasia-mutated (ATM) protein kinase exists as a dimer in the cell nucleus. Changes in DNA structure induced by genotoxic stress lead to activation of ATM and phosphorylation of S1981 in trans. Once S1981 is phosphorylated, the dimer dissociates and active ATM monomers signal to downstream targets.

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Citations

1. DEK is required for homologous recombination repair of DNA breaks
   Authors: EA Smith, B Gole, NA Willis, R Soria, LM Starnes, EF Krumpelbec, AG Jegga, AM Ali, H Guo, AR Meetei, PR Andreassen, F Kappes, LM Vinnedge, JA Daniel, R Scully, L Wiesmüller, SI Wells
   Sci Rep, 2017;7(0):44662.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination
   Authors: TL Clarke, MP Sanchez-Ba, K Chiang, JJ Reynolds, J Herrero-Ru, TM Bandeiras, PM Matias, SL Maslen, JM Skehel, GS Stewart, CC Davies
   Mol. Cell, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. DNA-PK-A candidate driver of hepatocarcinogenesis and tissue biomarker that predicts response to treatment and survival.
   Authors: Cornell L, Munck J, Alsinet C, Villanueva A, Ogle L, Willoughby C, Televantou D, Thomas H, Jackson J, Burt A, Newell D, Rose J, Manas D, Shapiro G, Curtin N, Reeves H
   Clin Cancer Res, 2015;21(4):925-33.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - paraffin


4. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
   Authors: Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T
   Nat Commun, 2014;2(0):4172.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


5. Direct activation of ATM by resveratrol under oxidizing conditions.
   Authors: Lee, Ji-Hoon, Guo, Zhi, Myler, Logan R, Zheng, Suting, Paull, Tanya T
   PLoS ONE, 2014;9(6):e97969.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Severe reaction to radiotherapy for breast cancer as the presenting feature of ataxia telangiectasia.
   Authors: Byrd PJ, Srinivasan V, Last JI
   Br. J. Cancer, 2012;106(2):262-8.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


7. Lymphoid tumours and breast cancer in ataxia telangiectasia; substantial protective effect of residual ATM kinase activity against childhood tumours.
   Authors: Reiman A, Srinivasan V, Barone G
   Br. J. Cancer, 2011;105(4):586-91.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. RAD51D protects against MLH1-dependent cytotoxic responses to O(6)-methylguanine.
   Authors: Rajesh P, Rajesh C, Wyatt MD
   DNA Repair (Amst.), 2010;9(4):458-67.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Inhibition of CHK1 kinase by Go6976 converts 8-chloro-adenosine-induced G2/M arrest into S arrest in human myelocytic leukemia K562 cells.
   Authors: Jia XZ, Yang SY, Zhou J, Li SY, Ni JH, An GS, Jia HT
   Biochem. Pharmacol., 2009;77(5):770-80.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


10. Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
    Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM
    J. Cell Biol., 2008;183(3):385-91.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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