抗Hsp27抗体(Anti-Hsp27, Human/Mouse, Rabbit-Poly antibody)
掲載日情報：2018/11/26 現在Webページ番号：27129
Hsp27に対する抗体(Anti-Hsp27, Human/Mouse, Rabbit-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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価格
[在庫・価格 ：2024年05月02日 14時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Hsp27, Human/Mouse, Rabbit-Poly |
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Anti-Human/Mouse HSP27 Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2024年05月02日 14時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Hsp27, Human/Mouse, Rabbit-Poly
文献数： 3
- 商品コード：AF1580
- メーカー：RSD
- 包装：50μg
- 価格：¥85,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：28 kDa heat shock protein Genbank No： 3315 Protein Accession No： P04792 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human/Mouse | 免疫動物 | Rabbit | ||||
| 交差性 | Human | 適用 | Simple Western,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
ニュース2020年9月15日号 p.16
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| 製品記事 |
抗HSP27(Heat shock Protein 27)抗体 |
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| 関連記事 | |||||||
Anti-Human/Mouse HSP27 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF1580-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
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Product Details
| Species Reactivity | Human, Mouse |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant human HSP27Accession # P04792 |
| Source | Polyclonal Rabbit IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects recombinant and endogenous human and mouse HSP27 in Western blots. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | See below |
| Simple Western | 0.5 µg/mL | See below |
| Knockout Validated | HSP27 is specifically detected in HeLa humancervical epithelial carcinoma parental cell line but is not detectable inHSP27 knockout HeLa cell line. | |
| Western Blot | |
|---|---|
 | Detection of Human/Mouse HSP27 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. |
| Simple Western | |
 | Detection of Human HSP27 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 31 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580). This experiment was conducted under reducing conditions and using the12-230 kDa separation system. |
| Simple Western | |
 | Detection of Mouse HSP27 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP27 at approximately 31 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
| Knockout Validated | |
 | Western Blot Shows Human HSP27Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse HSP27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1580) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # MAB5718) is shown as a loading control.. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
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Related Product & Information
| Background | HSP27 |
|---|---|
| background_content | Background: HSP27 Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Elevated levels of HSPs have been observed in association with ischemia/reperfusion, cancer, and chronic heart failure. HSP27, also known as HSPB1, is a member of the small heat shock protein family, which also includes HSP25 and the alpha -crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3. Full length human HSP27 shares 83% and 81% aa identity with mouse and rat HSP27, respectively. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
- Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression.
Authors: Banerjee S, Lin CF, Skinner KA, Schiffhauer LM, Peacock J, Hicks DG, Redmond EM, Morrow D, Huston A, Shayne M, Langstein HN, Miller-Graziano CL, Strickland J, O'Donoghue L, De AK
Cancer Res., 2011;71(2):318-27.
Species: Human
Sample Type: Whole Cells
Application: Flow - The heat shock response and chaperones/heat shock proteins in brain tumors: surface expression, release, and possible immune consequences.
Authors: Graner MW, Cumming RI, Bigner DD
J. Neurosci., 2007;27(42):11214-27.
Species: Human
Sample Type: Cell Lysates
Application: WB - Phosphatidylinositol 3-kinase/Akt plays a role in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts.
Authors: Takai S, Tokuda H, Matsushima-Nishiwaki R, Hanai Y, Kato K, Kozawa O
J. Cell. Biochem., 2006;98(5):1249-56.
Species: Mouse
Sample Type: Cell Lysates
Application: WB
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