抗IL-15 Receptor α抗体(Anti-IL-15 Receptor α, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha Gly33-Lys205Accession # Q60819
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL‑15 R alpha is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse (rm) IL-2 R beta, and rmIL-2 R gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑15 R alpha Fc Chimera (Catalog # 551-MR)
Flow Cytometry	2.5 µg/106 cells	EL‑4 mouse lymphoblast cell line
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Neutralization	Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 0.8 ng/mL Recombinant Human IL‑15.

Neutralization

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 R alpha Antibody. Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF551). The ND50 is typically 0.3-1.5 µg/mL.

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Related Product & Information

Long Name	Interleukin 15 Receptor alpha
Entrez Gene IDs	3601 (Human); 16169 (Mouse)
Background	IL-15 R alpha
background_content	Background: IL-15 R alpha Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

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Citations

1. Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
   Authors: Freeman B, Meyer C, Slifka M
   J Virol, 2014;88(13):7474-84.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


2. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
   Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
   PLoS Pathog., 2012;8(1):e1002481.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells.
   Authors: Muller J, Waldmann T, Kruhlak M, Dubois S
   J Biol Chem, 2012;287(48):40328-38.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


4. Immunological mechanisms and clinical implications of regulatory T cell deficiency in a systemic autoimmune disorder: roles of IL-2 versus IL-15.
   Authors: Yang CH, Tian L, Ling GS, Trendell-Smith NJ, Ma L, Lo CK, Stott DI, Liew FY, Huang FP
   Eur. J. Immunol., 2008;38(6):1664-76.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


5. Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15.
   Authors: McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS
   Exp. Hematol., 2008;36(5):598-608.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth.
   Authors: Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP
   Cancer Res., 2006;66(21):10497-504.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
   Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
   J. Immunol., 2004;172(3):1754-62.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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