抗Eotaxin-2抗体(Anti-Eotaxin-2, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2Ile24-Val119Accession # Q9JKC0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects CCL24/Eotaxin‑2/MPIF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Eotaxin-2 is observed, and less than 1% cross-reactivity with recombinant mouse Eotaxin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 528-MP)
Neutralization	Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.02 µg/mL Recombinant Mouse CCL24/Eotaxin‑2/MPIF‑2.

Neutralization

Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Mouse CCL24/Eotaxin‑2 Antibody. Recombinant Mouse CCL24/Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin‑2 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL24/Eotaxin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF528). The ND50 is typically 0.5-2.5 µg/mL.

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Related Product & Information

Entrez Gene IDs	6369 (Human); 56221 (Mouse)
Background	CCL24/Eotaxin-2/MPIF-2
background_content	Background: CCL24/Eotaxin-2/MPIF-2 Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.

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Citations

1. TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus
   Authors: Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson
   PLoS Pathog., 2017;13(7):e1006536.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


2. Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart
   Eur J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


3. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. CCR3 is a target for age-related macular degeneration diagnosis and therapy.
   Authors: Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J
   Nature, 2009;460(7252):225-30.
   Species: Mouse
   Sample Type: Vitreous Humor
   Application: Neut

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